一种针对二胺氧化酶的特异性过氧化物酶偶联活性染色法。
A specific peroxidase-coupled activity stain for diamine oxidases.
作者信息
Houen G, Leonardsen L
机构信息
Institute of Biochemical Genetics, Copenhagen, Denmark.
出版信息
Anal Biochem. 1992 Aug 1;204(2):296-9. doi: 10.1016/0003-2697(92)90241-x.
A specific peroxidase-coupled activity staining method for diamine oxidase (DAO) was developed. Diaminobenzidine was found to inhibit DAO and to give rise to unspecific staining. Among several other reagents 4-Cl-1-naphthol was found to be most suitable. Using specific activity staining DAO could be visualized in polyacrylamide gels as a high-molecular-weight complex, which could be dissociated by Tween 20 but not by NP-40, Triton X-100, or Chaps.
开发了一种用于二胺氧化酶（DAO）的特异性过氧化物酶偶联活性染色方法。发现二氨基联苯胺可抑制DAO并产生非特异性染色。在其他几种试剂中，发现4-氯-1-萘酚最为合适。使用特异性活性染色，DAO在聚丙烯酰胺凝胶中可显示为一种高分子量复合物，该复合物可被吐温20解离，但不能被NP-40、 Triton X-100或Chaps解离。
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