Ignesti G, Perretti M, Buffoni F
Departimento di Farmacologia Preclinica e Clinica, M. Aiazzi Mancini, Firenze, Italy.
Agents Actions. 1988 Aug;25(1-2):37-42. doi: 10.1007/BF01969091.
Treatment with Aroclor 1254 produces a decrease of diamine oxidase (DAO) activity in quail liver microsomes evaluated on deamination of 14C-putrescine and of histamine. The inhibition observed with the 14C-putrescine assay has peculiar behavior; it increase with the increase of 14C-putrescine concentrations until it produces values of extracted radioactivity which are less than the blanks which do not contain proteins. This effect is reserved by dicyclohexylammonium, an inhibitor of spermidine synthase (SPDS). This paper shows that SPDS rapidly decreases the putrescine concentration during the assay of DAO in tissues, particularly when SPDS is increased by induction with Arocolor.
用多氯联苯混合物1254处理后，在鹌鹑肝脏微粒体中，通过对14C-腐胺和组胺的脱氨作用评估，二胺氧化酶（DAO）活性降低。在14C-腐胺测定中观察到的抑制作用具有特殊行为；它随着14C-腐胺浓度的增加而增加，直到产生的提取放射性值低于不含蛋白质的空白对照值。这种效应被亚精胺合酶（SPDS）的抑制剂二环己基铵所逆转。本文表明，在组织中DAO测定过程中，SPDS会迅速降低腐胺浓度，特别是当通过多氯联苯诱导使SPDS增加时。
