Zweig M H, Csako G
Clinical Pathology Department, Warren G Magnuson Clinical Center, National Institutes of Health, Bethesda, MD 20892.
Ann Clin Biochem. 1992 Sep;29 ( Pt 5):551-5. doi: 10.1177/000456329202900511.
Recently, nonisotopic (often automated) immunoassays for measuring serum free thyroxin (FT4) have become available. Though more costly than radioimmunoassays, they are considerably more convenient. We studied the influence of endogenous albumin and thyroxin-binding globulin concentration on five automated, nonisotopic methods of measuring FT4 [Enzymun on ES300 (one-step), Stratus I and II (essentially two-step), Delfia (two-step), and IMx (two-step)] in a mixed patient population. We observed that they (a) are influenced very little by endogenous serum binding proteins and (b) seem to have sufficient within-run precision to justify performing single measurements on patients' specimens.
最近，用于测量血清游离甲状腺素（FT4）的非同位素（通常为自动化）免疫测定法已可使用。尽管它们比放射免疫测定法成本更高，但便利性却大大提高。我们在混合患者群体中研究了内源性白蛋白和甲状腺素结合球蛋白浓度对五种自动化非同位素FT4测量方法[ES300上的酶免疫测定法（一步法）、Stratus I和II（本质上是两步法）、Delfia（两步法）以及IMx（两步法）]的影响。我们观察到，它们（a）受内源性血清结合蛋白的影响非常小，并且（b）似乎具有足够的批内精密度，足以证明对患者标本进行单次测量是合理的。
