Weetall H H, Hertl W, Ward F B, Hersh L S
Clin Chem. 1982 Apr;28(4 Pt 1):666-71.
We describe a mathematical model for a single-tube enzyme immunoassay for free thyroxin (FT4), involving use of a thyroxin/horseradish peroxidase (EC 1.11.1.7) conjugate that does not interact with thyroxin-binding globulin. In the presence of serum two populations of unassociated, or free, immunologically active constituents are present: FT4 and the conjugate. The concentrations of the former are determined by the serum constituents and of the latter by the albumin concentration. When a small quantity of antibody is added, it reacts with the variable amount of FT4 and with the constant amount of the conjugate, thus giving a measure of the FT4. We constructed a mathematical model based on thermodynamic binding constants and adsorption data. The model gives satisfactory agreement with the experimental data under a variety of experimental conditions. Results for 19 patients' serum samples demonstrate the validity of the concept.
我们描述了一种用于游离甲状腺素（FT4）的单管酶免疫测定的数学模型，该模型涉及使用不与甲状腺素结合球蛋白相互作用的甲状腺素/辣根过氧化物酶（EC 1.11.1.7）偶联物。在血清存在的情况下，存在两种未结合的或游离的免疫活性成分：FT4和偶联物。前者的浓度由血清成分决定，后者的浓度由白蛋白浓度决定。当加入少量抗体时，它会与可变数量的FT4和恒定数量的偶联物发生反应，从而得出FT4的测量值。我们基于热力学结合常数和吸附数据构建了一个数学模型。该模型在各种实验条件下都与实验数据具有令人满意的一致性。19例患者血清样本的结果证明了这一概念的有效性。
