Watson N R, Peschke V M, Russell D A, Sachs M M
Department of Biology, Washington University, St. Louis, Missouri 63130.
Biochem Genet. 1992 Aug;30(7-8):371-83. doi: 10.1007/BF00569328.
Isozyme analysis of L-alanine:2-oxoglutarate aminotransferase (ALT) in maize indicates that there are three genes encoding this enzyme activity. Two of the gene products interact with each other to form heterodimers, while the third gene product does not interact with the other two. Another isozyme that appears after gel electrophoresis and ALT staining is shown to be glutamate dehydrogenase-1. Anaerobic treatment does not result in increased ALT levels, indicating that the previously reported increase in alanine levels caused by this treatment may be due to increases in the level of pyruvate, a substrate of ALT.
玉米中L-丙氨酸：2-酮戊二酸转氨酶（ALT）的同工酶分析表明，有三个基因编码这种酶活性。其中两个基因产物相互作用形成异二聚体，而第三个基因产物不与其他两个相互作用。凝胶电泳和ALT染色后出现的另一种同工酶被证明是谷氨酸脱氢酶-1。厌氧处理不会导致ALT水平升高，这表明先前报道的这种处理导致的丙氨酸水平升高可能是由于ALT的底物丙酮酸水平升高所致。
