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植物中的谷氨酸脱氢酶同工酶

Isoenzymes of glutamate dehydrogenase in plants.

作者信息

Yue S B

机构信息

Department of Botany, University of Hong Kong, Hong Kong, B.C.C.

出版信息

Plant Physiol. 1969 Mar;44(3):453-7. doi: 10.1104/pp.44.3.453.

DOI:10.1104/pp.44.3.453
PMID:16657083
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC396107/
Abstract

Glutamate dehydrogenase of several different plants was resolved by polyacrylamide gel electrophoresis into separate molecular forms and the isoenzymic patterns detected by the tetrazolium technique were compared. The number of isoenzymes and their electrophoretic mobilities varied among the different plants studied. The isoenzymes were found to have the same coenzyme specificity and to localize in the mitochondrial fraction of the cell in all the plants examined. Electrophoretic heterogeneity in tissue homogenates was observed in some of the plants studied. The pattern of isoenzymes of mungbean hypocotyl was followed and shown to change during germination.

摘要

通过聚丙烯酰胺凝胶电泳将几种不同植物的谷氨酸脱氢酶分离成不同的分子形式，并比较了用四唑技术检测到的同工酶模式。在所研究的不同植物中，同工酶的数量及其电泳迁移率各不相同。在所检测的所有植物中，发现同工酶具有相同的辅酶特异性，并定位于细胞的线粒体部分。在一些所研究的植物中，观察到组织匀浆中的电泳异质性。跟踪了绿豆下胚轴同工酶的模式，并表明其在萌发过程中会发生变化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f96b/396107/0dfb9a03baa1/plntphys00208-0137-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f96b/396107/49d2ad6eaec8/plntphys00208-0136-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f96b/396107/0dfb9a03baa1/plntphys00208-0137-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f96b/396107/49d2ad6eaec8/plntphys00208-0136-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f96b/396107/0dfb9a03baa1/plntphys00208-0137-a.jpg

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2
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2
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3
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本文引用的文献

1
Intracellular localization of nitrate reductase, nitrite reductase, and glutamic Acid dehydrogenase in green leaf tissue.硝酸还原酶、亚硝酸盐还原酶和谷氨酸脱氢酶在绿叶组织中的细胞内定位。
Plant Physiol. 1967 Feb;42(2):233-7. doi: 10.1104/pp.42.2.233.
2
MULTIPLE FORMS OF ENZYMES: TISSUE, ONTOGENETIC, AND SPECIES SPECIFIC PATTERNS.酶的多种形式：组织、个体发育和物种特异性模式。
Proc Natl Acad Sci U S A. 1959 May;45(5):753-63. doi: 10.1073/pnas.45.5.753.
3
Effect of glutamic acid on the formation of two glutamic acid dehydrogenases of Neurospora.
豌豆谷氨酸脱氢酶：分离线粒体中多种形式和谷氨酸形成的定位。
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Differential role of glutamate dehydrogenase in nitrogen metabolism of maize tissues.谷氨酸脱氢酶在玉米组织氮代谢中的差异作用。
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6
Glutamate dehydrogenase from pumpkin cotyledons: characterization and isoenzymes.南瓜子叶中的谷氨酸脱氢酶：特性与同工酶
Plant Physiol. 1972 Apr;49(4):550-4. doi: 10.1104/pp.49.4.550.
7
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In Vitro. 1973 Mar-Apr;8(5):347-52. doi: 10.1007/BF02619059.
8
Analysis of L-alanine:2-oxoglutarate aminotransferase isozymes in maize.玉米中L-丙氨酸：2-酮戊二酸氨基转移酶同工酶的分析
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谷氨酸对粗糙脉孢菌两种谷氨酸脱氢酶形成的影响。
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Fed Proc. 1962 Jan-Feb;21:57-64.
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6
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