Southgate K M, Davies M, Booth R F, Newby A C
Department of Cardiology, University of Wales College of Medicine, Heath Park, Cardiff, U.K.
Biochem J. 1992 Nov 15;288 ( Pt 1)(Pt 1):93-9. doi: 10.1042/bj2880093.
We investigated the influence of two structurally unrelated inhibitors of matrix-degrading metalloproteinases, Ro 31-4724 and Ro 31-7467, on the primary proliferation of smooth-muscle cells from rabbit aortic explants. Both agents inhibited proliferation in a concentration-dependent manner, but did not affect cell viability. Smooth-muscle cells grown out from explants secreted 95 kDa and 72 kDa gelatinase enzymes that were also inhibited in a concentration-dependent manner by Ro 31-4724 and Ro 31-7467. Interstitial collagenase and stromelysin were not detected. We conclude that metalloproteinases are likely to be involved in the initiation of smooth-muscle proliferation.
我们研究了两种结构不相关的基质降解金属蛋白酶抑制剂Ro 31-4724和Ro 31-7467对兔主动脉外植体平滑肌细胞原代增殖的影响。两种药物均以浓度依赖性方式抑制增殖，但不影响细胞活力。从外植体生长出的平滑肌细胞分泌95 kDa和72 kDa的明胶酶，Ro 31-4724和Ro 31-7467也以浓度依赖性方式抑制这些酶。未检测到间质胶原酶和基质溶解素。我们得出结论，金属蛋白酶可能参与平滑肌增殖的起始过程。
