Reidy M A, Silver M
Am J Pathol. 1985 Feb;118(2):173-7.
Rat aortas were denuded of endothelium by the use of catheters, which removed either a zone 10-15 cells wide or a zone 90-120 cells wide. Each animal received 3H-thymidine at 1, 16, and 24 hours before death, and groups of 3 animals were killed at 2, 3, 4, and 7 days after the small injury or 4, 5, 7, 14, and 42 days after the large injury. Scanning electron microscopy showed that the smaller injury was rehealed with endothelium by approximately 72 hours, and the large injury was repopulated by endothelium after 7-8 days. In aortas subjected to the 10-15-cell-wide injury, no intimal thickening nor intimal smooth-muscle proliferation was observed. After the larger injury, no obvious intimal thickening was present, although a few intimal cells were observed at sites where the internal elastic lamella was discontinuous. No significant increase in smooth-muscle-cell replication was detected in these aortas at any time after injury.
通过使用导管剥除大鼠主动脉内皮，导管移除的区域宽度为10 - 15个细胞或90 - 120个细胞。每组3只动物，在处死前1、16和24小时接受³H - 胸腺嘧啶核苷注射，在小面积损伤后2、3、4和7天或大面积损伤后4、5、7、14和42天处死动物。扫描电子显微镜显示，较小面积损伤后约72小时内皮重新愈合，大面积损伤后7 - 8天内皮重新形成。在接受10 - 15个细胞宽度损伤的主动脉中，未观察到内膜增厚或内膜平滑肌增殖。大面积损伤后，虽然在内弹性膜不连续的部位观察到少数内膜细胞，但未出现明显的内膜增厚。损伤后任何时间在这些主动脉中均未检测到平滑肌细胞复制显著增加。
