Trueheart J, Herskowitz I
Department of Biochemistry and Biophysics, University of California, San Francisco 94143.
J Bacteriol. 1992 Dec;174(23):7831-3. doi: 10.1128/jb.174.23.7831-7833.1992.
We have developed a cytoduction assay to measure cell fusion quantitatively in the basidiomycete corn smut fungus Ustilago maydis. This assay employs a mutation conferring resistance to oligomycin that exhibits non-Mendelian inheritance and presumably affects the mitochondrial genome. After auxotrophic olir cells are mixed with prototrophic olis cells, prototrophic olir cells can be detected at a significant frequency after several hours of incubation, reaching a maximum of 10% of the total prototrophs in the mixture after 18 h. We demonstrate that this cell fusion event occurs only if the mating partners have different alleles of the a mating-type locus and is not influenced by the b locus. These studies support the view that the a locus but not the b locus controls establishment of the filamentous, pathogenic state.
我们开发了一种细胞导入测定法，用于定量测量担子菌玉米黑粉菌（Ustilago maydis）中的细胞融合。该测定法采用了一种赋予对寡霉素抗性的突变，这种突变表现出非孟德尔遗传，并且可能影响线粒体基因组。将营养缺陷型olir细胞与原养型olis细胞混合后，孵育数小时后可以以显著频率检测到原养型olir细胞，18小时后达到混合物中原养型细胞总数的10%。我们证明，这种细胞融合事件仅在交配伙伴具有a交配型位点的不同等位基因时才会发生，并且不受b位点的影响。这些研究支持了这样一种观点，即a位点而非b位点控制丝状致病状态的建立。
