用于检测结核分枝杆菌酚糖脂PGL-Tb1和二酰海藻糖抗原的斑点酶联免疫吸附测定

DOT-ELISA for detection of phenolic glycolipid PGL-Tb1 and diacyl-trehalose antigens of Mycobacterium tuberculosis.

作者信息

Papa F, Luquin M, David H L

机构信息

Unité de la Tuberculose et des Mycobactéries, Institut Pasteur, Paris.

出版信息

Res Microbiol. 1992 Mar-Apr;143(3):327-31. doi: 10.1016/0923-2508(92)90024-i.

DOI:10.1016/0923-2508(92)90024-i

PMID:1448617

Abstract

A DOT-ELISA method for detection of 2,3-diacyl-trehalose (DAT, previously referred to as SL-IV antigen) and triglycosyl phenol phthiocerol di-mycocerosate (PGL-Tb1) antigens from Mycobacterium tuberculosis is described. The method enabled the detection of both antigens in 14 clinical isolates of M. tuberculosis from different geographic origins; the presence of the glycolipids was confirmed by chemical analysis. It was therefore concluded that the synthesis of both of these compounds is characteristic of the species.

摘要

描述了一种用于检测结核分枝杆菌中2,3-二酰基海藻糖（DAT，以前称为SL-IV抗原）和三糖基酚结核硬脂酸二霉菌酸酯（PGL-Tb1）抗原的斑点酶联免疫吸附测定（DOT-ELISA）方法。该方法能够检测来自不同地理来源的14株结核分枝杆菌临床分离株中的这两种抗原；通过化学分析证实了糖脂的存在。因此得出结论，这两种化合物的合成是该菌种的特征。