Watanabe M, Honda I, Kawajiri K, Niinuma S, Kudoh S, Minnikin D E
School of Pharmacology, Tokyo College of Pharmacy.
Res Microbiol. 1995 Nov-Dec;146(9):791-7. doi: 10.1016/0923-2508(96)81075-8.
Sera from tuberculosis (TB) patients and healthy controls were tested by ELISA for their antibody titres against the two major phenolic glycolipids (PGLs) of Mycobacterium tuberculosis, PGL-tbO (a 1:3 mixture of PGL-tb1 and its analogue whose phthiocerol moiety is phenolphthiotriol A) and PGL-tbK. Both PGL-tbs were shown to be specific to M. tuberculosis, and the profiles of serum anti-PGL-tbK titres revealed that PGL-tbK, like PGL-tb1, was fairly widely distributed among strains of M. tuberculosis. Even when these two PGL-tbs were used, however, the rate of ELISA-positives was not very high among TB patients, which is probably explained by the nature of the disease. Moreover, a considerable number of sera from healthy controls, especially from younger age groups, had high anti-PGL-tb titres, which implies that environmental exposure to M. tuberculosis is much higher than has been estimated from the actual TB cases. The ELISA system using these species-specific PGL-tb antigens may be useful for the survey of TB infection, since it gives more direct information on TB infection than the PPD skin test.
采用酶联免疫吸附测定（ELISA）检测肺结核（TB）患者和健康对照者血清中针对结核分枝杆菌两种主要酚糖脂（PGLs）的抗体滴度，这两种酚糖脂分别是PGL-tbO（PGL-tb1与其类似物的1:3混合物，其结核菌醇部分为酚酚酞三醇A）和PGL-tbK。结果表明，两种PGL-tb均对结核分枝杆菌具有特异性，血清抗PGL-tbK滴度曲线显示，PGL-tbK与PGL-tb1一样，在结核分枝杆菌菌株中分布相当广泛。然而，即便使用这两种PGL-tb，肺结核患者中ELISA阳性率也不是很高，这可能与该疾病的性质有关。此外，相当数量的健康对照者血清，尤其是年轻人群体的血清，具有较高的抗PGL-tb滴度，这意味着环境中结核分枝杆菌暴露程度远高于根据实际肺结核病例所估计的水平。使用这些种特异性PGL-tb抗原的ELISA系统可能有助于结核病感染调查，因为与结核菌素皮肤试验相比，它能提供更直接的结核病感染信息。
