Cho S N, Shin J S, Daffe M, Chong Y, Kim S K, Kim J D
Department of Microbiology, Yonsei University College of Medicine, Seoul, Republic of Korea.
J Clin Microbiol. 1992 Dec;30(12):3065-9. doi: 10.1128/jcm.30.12.3065-3069.1992.
A monoclonal antibody (MAbIII604) specific to phenolic glycolipid Tb (PGL-Tb), a Mycobacterium tuberculosis-specific antigen, was produced and used in the detection of the antigen. MAbIII604 reacted with the PGL-Tb antigen but not with other phenolic glycolipids from Mycobacterium leprae, M. bovis, and M. kansasii, thus indicating the specificity of the monoclonal antibody to PGL-Tb. A dot enzyme-linked immunosorbent assay with MAbIII604 was employed to detect the PGL-Tb antigen in lipids purified from M. tuberculosis clinical isolates. Of 50 isolates, 32 (64.0%) showed clear evidence of the PGL-Tb antigen by the dot enzyme-linked immunosorbent assay, but there were marked variations in the intensities and sizes of spots. This suggests differences in PGL-Tb antigen production among M. tuberculosis strains even when they are grown in the same culture media and conditions. This was most evident from the fact that in only eight (16.0%) of the isolates examined was the PGL-Tb antigen detectable by thin-layer chromatography, which is much less sensitive for the detection of glycolipid antigens. This study shows that monoclonal antibodies specific to PGL-Tb are useful in detecting the antigen in lipid extracts and that there is a marked variation in the PGL-Tb production among M. tuberculosis clinical isolates.
制备了一种针对结核分枝杆菌特异性抗原酚糖脂Tb（PGL-Tb）的单克隆抗体（MAbIII604），并将其用于该抗原的检测。MAbIII604与PGL-Tb抗原发生反应，但不与来自麻风分枝杆菌、牛分枝杆菌和堪萨斯分枝杆菌的其他酚糖脂发生反应，从而表明该单克隆抗体对PGL-Tb具有特异性。采用含MAbIII604的斑点酶联免疫吸附测定法检测从结核分枝杆菌临床分离株中纯化的脂质中的PGL-Tb抗原。在50株分离株中，有32株（64.0%）通过斑点酶联免疫吸附测定法显示出PGL-Tb抗原的明确证据，但斑点的强度和大小存在显著差异。这表明即使结核分枝杆菌菌株在相同的培养基和条件下生长，其PGL-Tb抗原产生也存在差异。从以下事实可以最明显地看出这一点：在所检测的分离株中，只有8株（16.0%）通过薄层色谱法可检测到PGL-Tb抗原，而薄层色谱法对糖脂抗原的检测灵敏度要低得多。本研究表明，针对PGL-Tb的单克隆抗体可用于检测脂质提取物中的抗原，并且结核分枝杆菌临床分离株之间的PGL-Tb产生存在显著差异。
