Ridell M, Wallerström G, Minnikin D E, Bolton R C, Magnusson M
Department of Medical Microbiology and Immunology, University of Gothenburg, Sweden.
Tuber Lung Dis. 1992 Apr;73(2):101-5. doi: 10.1016/0962-8479(92)90063-P.
Two fractions of antigenic diacyl trehaloses (DAT1 and DAT2) were isolated from the type strain of Mycobacterium tuberculosis (H37Rv). Phenolic glycolipid (PGL) and polar glycolipid antigens (C1-C4) were isolated from an unusual smooth so-called 'Canetti' strain of M. tuberculosis. These lipids were analyzed by enzyme-linked immunosorbent assay (ELISA) using antisera against a range of mycobacteria and sera from 50 tuberculosis patients and 25 healthy blood donors. All the lipids gave strong reactions with homologous mycobacterial antisera, except the least polar 'Canetti' polar glycolipid (C4). The phenolic glycolipid (PGL) from the 'Canetti' strain gave only a very weak response with antisera against M. tuberculosis H37Rv. The diacyl trehaloses (DAT) from H37Rv gave only weak reactions with the antisera against the 2 Canetti strains of M. tuberculosis. The 3 most polar glycolipid antigens (C1-C3) from the Canetti strain gave strong responses with serum against M. tuberculosis H37Rv. None of the lipids was able to discriminate between patient and control sera at a level suitable for a serodiagnostic test. A combination of results from several lipids appears to be of greater value in this respect. Thus, PGL, DAT2 and C2 were the best combination, reacting with all but 4 of the patient sera and with only 1 of the control sera.
从结核分枝杆菌（H37Rv）标准菌株中分离出两种抗原性二酰海藻糖组分（DAT1和DAT2）。从一株不寻常的光滑型所谓“卡内蒂”结核分枝杆菌菌株中分离出酚糖脂（PGL）和极性糖脂抗原（C1 - C4）。使用针对一系列分枝杆菌的抗血清以及来自50名结核病患者和25名健康献血者的血清，通过酶联免疫吸附测定（ELISA）对这些脂质进行分析。除了极性最小的“卡内蒂”极性糖脂（C4）外，所有脂质与同源分枝杆菌抗血清均产生强烈反应。“卡内蒂”菌株的酚糖脂（PGL）与抗结核分枝杆菌H37Rv的抗血清反应非常微弱。H37Rv的二酰海藻糖（DAT）与抗两种“卡内蒂”结核分枝杆菌菌株的抗血清反应微弱。“卡内蒂”菌株的3种极性最强的糖脂抗原（C1 - C3）与抗结核分枝杆菌H37Rv的血清反应强烈。在适合血清学诊断测试的水平上，没有一种脂质能够区分患者血清和对照血清。在这方面，几种脂质的结果组合似乎具有更大的价值。因此，PGL、DAT2和C2是最佳组合，与除4份患者血清外的所有患者血清反应，仅与1份对照血清反应。
