大肠杆菌的蔗糖依赖性壮观霉素抗性突变体。
Sucrose-dependent spectinomycin-resistant mutants of Escherichia coli.
作者信息
Miyoshi Y, Yamagata H
出版信息
J Bacteriol. 1976 Jan;125(1):142-8. doi: 10.1128/jb.125.1.142-148.1976.
Abstract
Spectinomycin-resistant (Spcr) mutants of Escherichia coli were isolated from nutrient agar plates containing 20% sucrose and 100 mug of spectinomycin per ml. About one-third of the Spcr mutants thus obtained were sucrose dependent (Sucd) and were classified into two types: I, those unable to grow on sucrose-free medium in the presence of spectinomycin; and II, those unable to grow on sucrose-free medium irrespective of the presence of spectinomycin. Most of these mutants were hypersensitive to antibiotics, dyes, and detergents and were abnormal in cell morphology, suggesting changes in cell envelopes. Reversion experiments indicated that the sucrose-dependent spectinomycin resistance and hypersensitivity to various chemicals were not independently induced properties. The Sucd-Spcr mutations of type I mutants were transducible by phage P1 and were mapped at the strA-aroE region.
摘要
从含有20%蔗糖和每毫升100微克壮观霉素的营养琼脂平板中分离出大肠杆菌的壮观霉素抗性（Spcr）突变体。由此获得的约三分之一的Spcr突变体依赖蔗糖（Sucd），并分为两种类型：I型，在有壮观霉素存在的情况下不能在无蔗糖培养基上生长；II型，无论有无壮观霉素都不能在无蔗糖培养基上生长。这些突变体大多对抗生素、染料和去污剂高度敏感，细胞形态异常，提示细胞包膜发生了变化。回复实验表明，蔗糖依赖性壮观霉素抗性和对各种化学物质的超敏感性不是独立诱导的特性。I型突变体的Sucd-Spcr突变可被噬菌体P1转导，并定位在strA-aroE区域。
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