Bilgin N, Richter A A, Ehrenberg M, Dahlberg A E, Kurland C G
Department of Molecular Biology, Uppsala University, Sweden.
EMBO J. 1990 Mar;9(3):735-9. doi: 10.1002/j.1460-2075.1990.tb08167.x.
We have compared the influence of spectinomycin (Spc) on individual partial reactions during the elongation phase of translation in vitro by wild-type and mutant ribosomes. The data show that the antibiotic specifically inhibits the elongation factor G (EF-G) cycle supported by wild-type ribosomes. In addition, we have reproduced the in vivo Spc resistant phenotype of relevant ribosome mutants in our in vitro translation system. In particular, three mutants with alterations at position 1192 in 16S rRNA as well as an rpsE mutant with an alteration of protein S5 were analysed. All of these ribosomal mutants confer a degree of Spc resistance for the EF-G cycle in vitro that is correlated with the degree of growth rate resistance to the antibiotic in culture.
我们比较了壮观霉素（Spc）对野生型和突变型核糖体体外翻译延伸阶段各个部分反应的影响。数据表明，该抗生素特异性抑制野生型核糖体支持的延伸因子G（EF-G）循环。此外，我们在体外翻译系统中重现了相关核糖体突变体的体内Spc抗性表型。特别分析了16S rRNA中第1192位发生改变的三个突变体以及蛋白质S5发生改变的rpsE突变体。所有这些核糖体突变体在体外对EF-G循环都具有一定程度的Spc抗性，这与培养物中对抗生素的生长速率抗性程度相关。
