Brunngraber E F, Chargaff E
Proc Natl Acad Sci U S A. 1977 Oct;74(10):4160-2. doi: 10.1073/pnas.74.10.4160.
The action of the nucleotide phosphotransferase of Escherichia coli on nicotinamide riboside and on its 5'-phosphate results in the addition of one phosphate moiety to each of the substrates. Although the proof is not conclusive, it is likely that the phosphate group is transferred to the 3'-hydroxyl of the ribose. This is in contrast to the behavior of the enzyme toward NAD in which only the adenylic acid portion is phosphorylated enzymically.
大肠杆菌的核苷酸磷酸转移酶作用于烟酰胺核糖及其5'-磷酸酯,会使每个底物都添加一个磷酸基团。虽然证据并不确凿,但磷酸基团很可能转移到了核糖的3'-羟基上。这与该酶对NAD的作用不同,在对NAD的作用中,只有腺苷酸部分会被酶促磷酸化。