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大肠杆菌核苷酸磷酸转移酶对烟酰胺核糖及烟酰胺单核苷酸的作用。

Action of nucleotide phosphotransferase of Escherichia coli on nicotinamide riboside and nicotinamide mononucleotide.

作者信息

Brunngraber E F, Chargaff E

出版信息

Proc Natl Acad Sci U S A. 1977 Oct;74(10):4160-2. doi: 10.1073/pnas.74.10.4160.

DOI:10.1073/pnas.74.10.4160
PMID:144913
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC431897/
Abstract

The action of the nucleotide phosphotransferase of Escherichia coli on nicotinamide riboside and on its 5'-phosphate results in the addition of one phosphate moiety to each of the substrates. Although the proof is not conclusive, it is likely that the phosphate group is transferred to the 3'-hydroxyl of the ribose. This is in contrast to the behavior of the enzyme toward NAD in which only the adenylic acid portion is phosphorylated enzymically.

摘要

大肠杆菌的核苷酸磷酸转移酶作用于烟酰胺核糖及其5'-磷酸酯,会使每个底物都添加一个磷酸基团。虽然证据并不确凿,但磷酸基团很可能转移到了核糖的3'-羟基上。这与该酶对NAD的作用不同,在对NAD的作用中,只有腺苷酸部分会被酶促磷酸化。

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本文引用的文献

1
Paper chromatography of nicotinic acid derivatives.烟酸衍生物的纸色谱法。
Nature. 1951 Sep 15;168(4272):475-7. doi: 10.1038/168475b0.
2
The reaction of pyridine nucleotide with cyanide and its analytical use.吡啶核苷酸与氰化物的反应及其分析应用。
J Biol Chem. 1951 Aug;191(2):447-59.
3
On the structure of triphosphopyridine nucleotide.论三磷酸吡啶核苷酸的结构。
J Biol Chem. 1950 Oct;186(2):557-67.
4
A specific and nonspecific alkaline monophosphatase in the venom of Bothrops atrox and their occurrence in the purified venom phosphodiesterase.矛头蝮蛇毒中的一种特异性和非特异性碱性单磷酸酶及其在纯化的蛇毒磷酸二酯酶中的存在情况。
J Biol Chem. 1963 Jul;238:2477-86.
5
Studies on the nucleoside phosphotransferase of carrot. III. On the synthetic ability of the transfer enzyme.胡萝卜核苷磷酸转移酶的研究。III. 关于转移酶的合成能力。
Biochim Biophys Acta. 1960 May 20;40:206-10. doi: 10.1016/0006-3002(60)91343-3.
6
A specific b nucleotidase.一种特定的b核苷酸酶。
J Biol Chem. 1953 Apr;201(2):535-46.
7
Purification and properties of a nucleoside phosphotransferase from carrot.胡萝卜中一种核苷磷酸转移酶的纯化及性质
J Biol Chem. 1967 Oct 25;242(20):4834-40.
8
Transferase from Escherichia coli effecting low-energy phosphate transfer to nucleosides and nucleotides.来自大肠杆菌的转移酶,可实现低能磷酸向核苷和核苷酸的转移。
Proc Natl Acad Sci U S A. 1970 Sep;67(1):107-12. doi: 10.1073/pnas.67.1.107.
9
A nucleotide phosphotransferase from Escherichia coli. Purification and properties.来自大肠杆菌的一种核苷酸磷酸转移酶。纯化及特性
Biochemistry. 1973 Jul 31;12(16):3005-12. doi: 10.1021/bi00740a009.
10
Nicotinamide adenine dinucleotide as substrate of the nucleotide phosphotransferase from Escherichia coli.
Biochemistry. 1973 Jul 31;12(16):3012-6. doi: 10.1021/bi00740a010.