Weaver S C, Lorenz L H, Scott T W
Department of Entomology, University of Maryland, College Park.
Am J Trop Med Hyg. 1992 Nov;47(5):691-701. doi: 10.4269/ajtmh.1992.47.691.
Midguts of two strains of the mosquito vector Culex tarsalis were examined by light and electron microscopy following infection with western equine encephalomyelitis virus. Infection of the highly susceptible Knight's Landing strain with high-titered blood meals resulted in pathologic changes in the midgut epithelium after 2-4 days of incubation; lesions included sloughing of epithelial cells into the lumen and necrosis of cells in situ. Infection of Knight's Landing strain mosquitoes with low-titered blood meals and infection of the less susceptible Fort Collins strain with high-titered blood meals did not result in a significant increase in detached luminal cells, with respect to uninfected controls. Sloughing of infected cells into the midgut lumen may contribute to modulation of the mosquito infection. Lesions in the midgut of Cx. tarsalis are inconsistent with traditional views that regarded arbovirus infections of mosquito vectors as non-pathologic. These findings demonstrate that mosquito pathology is not an oddity limited to the previously described interaction between Culiseta melanura and eastern equine encephalomyelitis virus, and suggest that alphaviruses in general may adversely affect their mosquito vectors in nature.
在用西部马脑炎病毒感染之后，通过光学显微镜和电子显微镜对两种库蚊属媒介——致倦库蚊的中肠进行了检查。用高滴度血餐感染高度易感的奈茨登陆品系，在孵育2 - 4天后中肠上皮出现病理变化；病变包括上皮细胞脱落至肠腔以及原位细胞坏死。用低滴度血餐感染奈茨登陆品系的蚊子以及用高滴度血餐感染易感程度较低的柯林斯堡品系，与未感染对照相比，肠腔中脱落细胞数量并未显著增加。受感染细胞脱落至中肠肠腔可能有助于调节蚊子的感染情况。致倦库蚊中肠的病变与传统观点不一致，传统观点认为虫媒病毒对蚊媒的感染是非病理性的。这些发现表明，蚊子的病理学变化并非仅限于先前描述的黑尾脉毛蚊与东部马脑炎病毒之间的相互作用这种特殊情况，并且提示一般而言甲病毒在自然界中可能会对其蚊媒产生不利影响。
