Sazuka T, Tomooka Y, Ikawa Y, Noda M, Kumar S
Laboratory of Molecular Oncology, RIKEN, Tsukuba Life Science Center, Ibaraki, Japan.
Biochem Biophys Res Commun. 1992 Nov 30;189(1):363-70. doi: 10.1016/0006-291x(92)91567-a.
Using a subtraction cloning approach we had previously isolated a series of murine cDNA clones representing the genes predominantly expressed in the embryonic brain and down-regulated during development. We now report that one of these cDNA clones encodes a novel type of GTP-binding protein. The predicted protein of 40.5 kD, named DRG, contains five structural motifs characteristic of the GTP-binding proteins. Consistently, bacterially expressed and cellular DRG proteins are capable of binding GTP in vitro. Sequences closely related to the DRG protein are found in other species including Drosophila and Halobacterium. Based on these observations, we propose that DRG represents an evolutionarily conserved novel class of GTP-binding protein which may play an important role in cell physiology.
我们先前采用消减克隆方法分离出了一系列小鼠cDNA克隆，这些克隆代表了在胚胎脑中主要表达且在发育过程中表达下调的基因。我们现在报告，其中一个cDNA克隆编码一种新型GTP结合蛋白。预测的40.5 kD蛋白名为DRG，含有GTP结合蛋白特有的五个结构基序。一致地，细菌表达的和细胞内的DRG蛋白在体外能够结合GTP。在包括果蝇和盐杆菌在内的其他物种中发现了与DRG蛋白密切相关的序列。基于这些观察结果，我们提出DRG代表了一类进化上保守的新型GTP结合蛋白，其可能在细胞生理学中起重要作用。
