Zhao X F, Aplan P D
Departments of Pediatrics and Molecular Medicine, Roswell Park Cancer Institute, Elm and Carlton Streets, Buffalo, NY 14263, USA.
Biochim Biophys Acta. 1998 Nov 19;1448(1):109-14. doi: 10.1016/s0167-4889(98)00129-3.
A suspected oncoprotein, human development regulated GTP-binding protein (DRG) has never been identified though homologues were found in mouse, Xenopus, Drosophila, yeast and Halobacteria. During a search for SCL binding partners using the yeast 2-hybrid system, we isolated two independent cDNA clones (clone L51 and clone V3) of the human DRG homologue from human fetal liver and human thymus cDNA libraries. Only one amino acid difference was found between human and mouse DRG proteins. Although a human DRG has been previously deposited in the SWISS-PROT Database, we believe that we have cloned the bona fide human DRG based on the highly conserved primary amino acid structure between our cloned human homologue and the mouse DRG.
一种疑似癌蛋白，即人类发育调控性GTP结合蛋白（DRG），尽管在小鼠、非洲爪蟾、果蝇、酵母和嗜盐菌中发现了其同源物，但从未被鉴定出来。在利用酵母双杂交系统寻找SCL结合伴侣的过程中，我们从人胎儿肝脏和人胸腺cDNA文库中分离出了两个独立的人类DRG同源物cDNA克隆（克隆L51和克隆V3）。人类和小鼠的DRG蛋白之间仅发现一个氨基酸差异。尽管之前已有一种人类DRG存入了SWISS-PROT数据库，但基于我们克隆的人类同源物与小鼠DRG之间高度保守的一级氨基酸结构，我们认为我们克隆到了真正的人类DRG。
