Santos Terezinha de Jesus Teixeira, Costa Carlos Maurício de Castro, Goubau Patrick, Vandamme Anne-Mieke, Desmyter Jan, Van Doren Sonia, Mota Rosa M S, de Castro Costa Francine Bovy, Oliveira Ana C S, Barreto Vania, Gomes A F, Carneiro-Proietti Anna B, de Bruin Veralice Meireles Sales, de Sousa Francisca C F, Oriá Reinaldo Barreto
Department of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, CE, Brazil.
Braz J Infect Dis. 2003 Jun;7(3):202-9. doi: 10.1590/s1413-86702003000300005.
How to handle Western blot (WB) seroindeterminate individuals for Human T-lymphotropic Virus 1/2 (HTLV-1/2) constitutes a challenge for blood banks and families. We made a cross-sectional study of 191 enzyme linked immunoassay (EIA) reactive individuals from the hematological center (HEMOCE) of Fortaleza (Brazil), examining their serological (WB) and molecular (PCR) diagnosis, and demographic profiles, as well as a possible association of their condition with other infectious pathologies and risk factors. Ethical institutional approval and personal consent were obtained. Out of 191 EIA reactive individuals, 118 were WB seroindeterminate and 73 were seropositive for HTLV-1/2. In the PCR analysis of 41 WB seroindeterminate individuals, 9 (22%) were positive and 32 (78%) were negative for HTLV-1/2. The demographic analysis indicated a trend towards a predominance of males among the seroindeterminate individuals and females in the seropositive ones. The seroindeterminate individuals were younger than the seropositive ones. We did not find any association of these conditions with syphilis, Chagas disease or HIV or hepatitis, and with risk factors such as breast-feeding, blood transfusion, STD (syphilis) and IDU.
如何处理人类嗜T淋巴细胞病毒1/2（HTLV-1/2）免疫印迹（WB）血清学检测结果不确定的个体，这对血库和患者家庭来说是一项挑战。我们对来自巴西福塔莱萨血液学中心（HEMOCE）的191名酶联免疫吸附测定（EIA）呈反应性的个体进行了横断面研究，检测了他们的血清学（WB）和分子（PCR）诊断结果、人口统计学特征，以及他们的病情与其他感染性疾病和风险因素之间可能存在的关联。研究获得了机构伦理批准和个人同意。在191名EIA呈反应性的个体中，118人WB血清学检测结果不确定，73人HTLV-1/2血清学检测呈阳性。在对41名WB血清学检测结果不确定的个体进行的PCR分析中，9人（22%）HTLV-1/2呈阳性，32人（78%）呈阴性。人口统计学分析表明，血清学检测结果不确定的个体中男性占主导的趋势明显，而血清学检测呈阳性的个体中女性居多。血清学检测结果不确定的个体比血清学检测呈阳性的个体更年轻。我们没有发现这些情况与梅毒、恰加斯病、艾滋病毒或肝炎，以及母乳喂养、输血、性传播疾病（梅毒）和注射吸毒等风险因素之间存在任何关联。
