Research Centre for HIV/AIDS, HTLV and Viral Hepatitis, Iranian Academic Centre for Education, Culture & Research (ACECR), Mashhad Branch, Mashhad, Iran ; Inflammation and Inflammatory Diseases Research Centre, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Research Centre for HIV/AIDS, HTLV and Viral Hepatitis, Iranian Academic Centre for Education, Culture & Research (ACECR), Mashhad Branch, Mashhad, Iran.
Iran J Basic Med Sci. 2013 Mar;16(3):229-34.
OBJECTIVE(S): Although HTLV-I infection is endemic in different geographical parts of the world including Northeast of Iran, there have been no documents of HTLV-II infection in this region. It is reported that one possible reason for seroindeterminate state in HTLV western blot is HTLV-II virus. This study aimed to investigate the presence of HTLV-II among blood donors with seroindeterminate western blot results.
Three ml whole blood obtained from 50 blood donors referring to Mashhad Blood Transfusion Organization who had reactive Elisa for HTLV-I and seroindeterminate HTLV western blot state. A conventional PCR was applied to detect HTLV-I provirus using specific primers while a nested PCR was designed with specific external and internal primers for the detection of HTLV-II.
The average age of participants, 39 males and 11 females, was 37.12± 14.36 years. The average OD of the Elisa assay was 1.767± 1.195. The most common indeterminate patterns were Rgp46-II alone (n=12, 27.3%), Rgp46-I alone (n=7, 15.9%), and Rgp46-I with GD21 (n=7, 15.9%).After introducing the DNA to the PCR tests, results revealed 10 (20%) HTLV-I PCR positive samples while no HTLV-II positive sample was detected by nested PCR. There were no significant age, blood group, Optical Density of the Elisa assay, and western blot indeterminate pattern differences between HTLV-I PCR positive and negative samples. Conclusion : No HTLV-II positive sample was detected in this study which confirms the absence of HTLV-II infection in this region. However, high frequency of HTLV-I PCR positive samples among the seroindeterminate cases implies on the important role of molecular techniques for further confirmation of the infection.
尽管 HTLV-I 感染在包括伊朗东北部在内的世界不同地理区域流行，但该地区尚未有 HTLV-II 感染的记录。据报道，HTLV 免疫印迹出现血清不确定状态的一个可能原因是 HTLV-II 病毒。本研究旨在调查血清学不确定的 HTLV 免疫印迹结果的献血者中是否存在 HTLV-II。
从马什哈德血液中心的 50 名 HTLV-I 酶联免疫吸附试验（ELISA）阳性且 HTLV 免疫印迹血清不确定的献血者中采集 3ml 全血。使用特异性引物的常规聚合酶链反应（PCR）用于检测 HTLV-I 前病毒，而设计了巢式 PCR 以使用特异性外部和内部引物检测 HTLV-II。
39 名男性和 11 名女性参与者的平均年龄为 37.12±14.36 岁。ELISA 检测的平均 OD 值为 1.767±1.195。最常见的不确定模式是单独的 Rgp46-II（n=12，27.3%）、单独的 Rgp46-I（n=7，15.9%）和 Rgp46-I 与 GD21（n=7，15.9%）。将 DNA 引入 PCR 检测后，结果显示 10（20%）例 HTLV-I PCR 阳性样本，而巢式 PCR 未检测到 HTLV-II 阳性样本。HTLV-I PCR 阳性和阴性样本之间在年龄、血型、ELISA 检测的光密度和免疫印迹不确定模式方面无显著差异。结论：本研究未检测到 HTLV-II 阳性样本，证实该地区不存在 HTLV-II 感染。然而，血清学不确定病例中 HTLV-I PCR 阳性样本的高频率表明分子技术对于进一步确认感染具有重要作用。
