表达来自树莓（悬钩子属）的多聚半乳糖醛酸酶抑制蛋白和来自葡萄（葡萄属）的芪合酶的转基因豌豆（豌豆）。
Transgenic peas (Pisum sativum) expressing polygalacturonase inhibiting protein from raspberry (Rubus idaeus) and stilbene synthase from grape (Vitis vinifera).
作者信息
Richter A, Jacobsen H-J, de Kathen A, de Lorenzo G, Briviba K, Hain R, Ramsay G, Kiesecker H
机构信息
Department of Molecular Genetics, University of Hannover, Herrenhäuserstr 2, 30419, Hannover, Germany.
出版信息
Plant Cell Rep. 2006 Nov;25(11):1166-73. doi: 10.1007/s00299-006-0172-z. Epub 2006 Jun 27.
The pea (Pisum sativum L.) varieties Baroness (United Kingdome) and Baccara (France) were transformed via Agrobacterium tumefaciens-mediated gene transfer with pGPTV binary vectors containing the bar gene in combination with two different antifungal genes coding for polygalacturonase-inhibiting protein (PGIP) from raspberry (Rubus idaeus L.) driven by a double 35S promoter, or the stilbene synthase (Vst1) from grape (Vitis vinifera L.) driven by its own elicitor-inducible promoter. Transgenic lines were established and transgenes combined via conventional crossing. Resveratrol, produced by Vst1 transgenic plants, was detected using HPLC and the PGIP expression was determined in functional inhibition assays against fungal polygalacturonases. Stable inheritance of the antifungal genes in the transgenic plants was demonstrated.
豌豆（Pisum sativum L.）品种“男爵夫人”（英国）和“巴卡拉”（法国）通过根癌农杆菌介导的基因转移，用含有bar基因的pGPTV双元载体进行转化，该载体与两个不同的抗真菌基因组合，其中一个抗真菌基因编码由双35S启动子驱动的来自树莓（Rubus idaeus L.）的多聚半乳糖醛酸酶抑制蛋白（PGIP），另一个抗真菌基因编码由其自身诱导子诱导型启动子驱动的来自葡萄（Vitis vinifera L.）的芪合酶（Vst1）。建立了转基因株系，并通过常规杂交将转基因进行组合。使用高效液相色谱法检测Vst1转基因植物产生的白藜芦醇，并在针对真菌多聚半乳糖醛酸酶的功能抑制试验中测定PGIP的表达。证明了抗真菌基因在转基因植物中的稳定遗传。
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