Xu Yuanyuan, Du Wei, Liu Dehua, Zeng Jing
Department of Chemical Engineering, Tsinghua University, Beijing 100084, PR China.
Biotechnol Lett. 2003 Aug;25(15):1239-41. doi: 10.1023/a:1025065209983.
A new enzymatic route for biodiesel production from soybean oil was developed using methyl acetate as a novel acyl acceptor. Novozym 435 (immobilized Candida antarctica lipase) gave the highest methyl ester (ME) yield of 92%. The optimum conditions of the transesterification were 30% enzyme based on oil weight; a molar ratio of methyl acetate/oil of 12:1; temperature 40 degrees C and reaction time 10 h. Since no glycerol was produced in the process, this method is very convenient for recycling the catalyst and by-product triacetylglycerol showed no negative effect on the fuel property.
开发了一种以乙酸甲酯作为新型酰基受体从大豆油生产生物柴油的新酶促路线。诺维信435（固定化南极假丝酵母脂肪酶）甲酯（ME）产率最高，为92%。酯交换反应的最佳条件为：酶用量为油重的30%；乙酸甲酯与油的摩尔比为12:1；温度40℃，反应时间10小时。由于该过程中不产生甘油，此方法对于催化剂回收非常方便，且副产物三乙酰甘油对燃料性能无负面影响。
