Green Processes Center, Chemical Engineering Department, Sami Shamoon College of Engineering, Bialik/Basel Sts., 84100 Beer Sheva, Israel.
Bioprocess Biosyst Eng. 2010 Mar;33(3):363-6. doi: 10.1007/s00449-009-0333-x. Epub 2009 May 28.
Glycerol triacetate was successfully used as a green solvent and as the acyl donor in the transesterification of isoamyl alcohol to produce isoamyl acetate using free and immobilized Candida antarctica lipase B. Immobilized lipase was more catalytically active than free lipase and could be easily separated from the reaction mixture by filtration. In addition, it was found that increasing either the reaction temperature or the enzyme to substrate ratio increased the conversion of isoamyl alcohol. Using triacetin as the solvent also enabled the separation of product by simple extraction with petroleum ether and catalyst recycling.
三醋酸甘油酯成功地被用作一种绿色溶剂和酰基供体，在游离和固定化南极假丝酵母脂肪酶 B 的作用下，用于异戊醇的酯交换反应以生产乙酸异戊酯。固定化脂肪酶比游离脂肪酶具有更高的催化活性，并且可以通过过滤从反应混合物中轻易分离。此外，研究还发现，升高反应温度或酶与底物的比例都能提高异戊醇的转化率。使用三醋酸甘油酯作为溶剂还可以通过简单的石油醚萃取和催化剂回收来分离产物。
