采用双细胞角蛋白和CD31免疫组织化学检测肿瘤淋巴管间隙浸润
Detection of tumour lymphovascular space invasion using dual cytokeratin and CD31 immunohistochemistry.
作者信息
Alexander-Sefre F, Singh N, Ayhan A, Salveson H B, Wilbanks G, Jacobs I J
机构信息
Gynaecological Cancer Research Centre, St Bartholomew's and The London School of Medicine, London EC1M 6BQ, UK.
出版信息
J Clin Pathol. 2003 Oct;56(10):786-8. doi: 10.1136/jcp.56.10.786.
Abstract
BACKGROUND
Lymphovascular space invasion (LVSI) is an important step in the complex process of tumour metastasis. Various methods have been used in the past to improve the histological detection of LVSI.
AIMS
To develop a sensitive immunohistochemical method for the detection of LVSI.
METHODS
Paraffin wax blocks from 108 patients who had undergone hysterectomy for stage I endometrial cancer were retrieved. Dual immunostaining for pancytokeratin and the CD31 endothelial cell marker was carried out on 4 micro m sections cut from these bocks and compared with conventional haematoxylin and eosin staining.
RESULTS
The detection rate for LVSI increased threefold compared with conventional haematoxylin and eosin staining in the test group.
CONCLUSION
This finding suggests that LVSI is a much more common phenomenon than previously thought and questions current understanding of tumour metastasis.
摘要
背景
淋巴管血管间隙浸润（LVSI）是肿瘤转移复杂过程中的重要一步。过去已采用多种方法来提高LVSI的组织学检测水平。
目的
开发一种检测LVSI的敏感免疫组织化学方法。
方法
检索108例因I期子宫内膜癌接受子宫切除术患者的石蜡块。从这些石蜡块切取的4微米切片上进行全细胞角蛋白和CD31内皮细胞标志物的双重免疫染色，并与传统苏木精和伊红染色进行比较。
结果
试验组中LVSI的检出率比传统苏木精和伊红染色提高了两倍。
结论
这一发现表明LVSI是一种比之前认为的更为常见的现象，并对当前对肿瘤转移的认识提出了质疑。
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