Ortiz Cesar Mateo Flores, Castro Ignacio Peñalosa, Portilla Luis Barbo Hernández, Aranda Patricia Dolores Dávila, Arizmendi Maria del Coro
Laboratorio de Fisiología Vegetal, UBIPRO, Facultad de Estudios Superiores Iztacala, UNAM, México.
Phytochem Anal. 2003 Sep-Oct;14(5):319-24. doi: 10.1002/pca.723.
An instrumental method based on a chemometric model of the medium region of the infrared (MIR) was developed to analyse total sugar content and the proportions of glucose, fructose and sucrose. In order to construct the model, a set of 127 standard aqueous solutions of different sugars in the concentration range 0-20% (w/v) were prepared and analysed in the interval 4900-700 cm(-1). The MIR was transformed by normalisation, correction of baseline using the second derivative, and suppression of the signals of water and carbon dioxide. The region between 1150 and 950 cm(-1) showed the highest correlation between signal and concentration. The correlation coefficient for total sugar content was 0.956, whilst those for glucose, fructose and sucrose were 0.982, 0.972 and 0.992, respectively. The method was validated using a set of 28 samples of nectar which had been assayed by chromatographic and refractometric methods. The method shows potential utility for the prediction of nectar sugar components.
开发了一种基于中红外（MIR）介质区域化学计量模型的仪器方法，用于分析总糖含量以及葡萄糖、果糖和蔗糖的比例。为构建该模型，制备了一组127种不同糖类的标准水溶液，浓度范围为0 - 20%（w/v），并在4900 - 700 cm⁻¹区间进行分析。通过归一化、使用二阶导数校正基线以及抑制水和二氧化碳信号对MIR进行了变换。1150至950 cm⁻¹之间的区域显示出信号与浓度之间的最高相关性。总糖含量的相关系数为0.956，而葡萄糖、果糖和蔗糖的相关系数分别为0.982、0.9７2和0.992。使用一组已通过色谱和折光法测定的28个花蜜样品对该方法进行了验证。该方法在预测花蜜糖成分方面显示出潜在的实用性。
