一种氨基酸对转录因子功能的调节：脯氨酸对Put3p的激活作用。
Modulation of transcription factor function by an amino acid: activation of Put3p by proline.
作者信息
Sellick Christopher A, Reece Richard J
机构信息
School of Biological Sciences, The University of Manchester, 2.205 Stopford Building, Oxford Road, Manchester M13 9PT, UK.
出版信息
EMBO J. 2003 Oct 1;22(19):5147-53. doi: 10.1093/emboj/cdg480.
Abstract
Saccharomyces cerevisiae are able to convert proline to glutamate so that it may be used as a source of nitrogen. Here, we show that the activator of the proline utilization genes, Put3p, is transcriptionally inert in the absence of proline but transcriptionally active in its presence. The activation of Put3p requires no additional yeast proteins and can occur in the presence of certain proline analogues: an unmodified pyrrolidine ring is able to activate Put3p as efficiently as proline itself. In addition, we show that a direct interaction occurs between Put3p and proline. These data, which represent direct control of transcriptional activator function by a metabolite, are discussed in terms of the regulation of proline-specific genes in yeast and as a general mechanism of the control of transcription.
摘要
酿酒酵母能够将脯氨酸转化为谷氨酸，以便将其用作氮源。在此，我们表明脯氨酸利用基因的激活因子Put3p在无脯氨酸时转录无活性，但在有脯氨酸时转录有活性。Put3p的激活不需要其他酵母蛋白，并且在某些脯氨酸类似物存在的情况下即可发生：未修饰的吡咯烷环激活Put3p的效率与脯氨酸本身相同。此外，我们表明Put3p与脯氨酸之间存在直接相互作用。这些代表代谢物对转录激活因子功能进行直接调控的数据，将从酵母中脯氨酸特异性基因的调控以及转录控制的一般机制方面进行讨论。
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