van Bokhoven Adrie, Varella-Garcia Marileila, Korch Christopher, Johannes Widya U, Smith E Erin, Miller Heidi L, Nordeen Steven K, Miller Gary J, Lucia M Scott
Department of Pathology, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA.
Prostate. 2003 Nov 1;57(3):205-25. doi: 10.1002/pros.10290.
This study presents a comprehensive survey and characterization of available prostate carcinoma cell lines, most of which have been widely used but are incompletely characterized.
A total of 21 cell lines were investigated, including three "classical" (DU 145, LNCaP, and PC-3) and 18 "non-classical" lines (1013L, 22Rv1, ALVA-55, ALVA-101, ARCaP, CWR-R1, DuCaP, DuPro-1, LAPC-4, MDA PCa 1, MDA PCa 2a, MDA PCa 2b, NCI-H660, PC-346C, PC-93, PSK-1, UM-SCP-1, and VCaP). Cytogenetics, DNA profiling, expression of basal, luminal, and neuroendocrine differentiation markers, and mutation analyses of the TP53 and androgen receptor (AR) genes were performed.
Based on cytogenetics and DNA profiling analyses, out of the 18 "non-classical" lines, six were confirmed to be unique, eight (in four pairs) were confirmed to be related in origin, and four lines were identified as cross-contaminants. Of this latter group, PC-93 was found to be a derivative of HeLa, whereas DuPro-1, ALVA-55, and ALVA-101 were derivatives of PC-3. The 17 genuine prostate cell lines expressed keratin 8 (K8) and K18. Nine showed AR expression, of which five harbored mutations in the AR gene. Prostate-specific antigen and DD3 were exclusively detected in AR expressing cell lines. Seven lines expressed the basal cell marker K5, three of these lines showed co-expression of AR.
This study defines a collection of 17 genuine prostate carcinoma cell lines. This collection, although small, constitutes a variety of different types and stages of prostate cancer, while it also partly reflects the heterogeneous nature of this malignancy.
本研究对现有的前列腺癌细胞系进行了全面的调查和特征描述，其中大多数细胞系已被广泛使用，但特征描述并不完整。
共研究了21种细胞系，包括三种“经典”细胞系（DU 145、LNCaP和PC-3）以及18种“非经典”细胞系（1013L、22Rv1、ALVA-55、ALVA-101、ARCaP、CWR-R1、DuCaP、DuPro-1、LAPC-4、MDA PCa 1、MDA PCa 2a、MDA PCa 2b、NCI-H660、PC-346C、PC-93、PSK-1、UM-SCP-1和VCaP）。进行了细胞遗传学、DNA谱分析、基底、管腔和神经内分泌分化标志物的表达分析以及TP53和雄激素受体（AR）基因的突变分析。
基于细胞遗传学和DNA谱分析，在18种“非经典”细胞系中，六种被确认为独特的，八种（四对）被确认为起源相关，四种细胞系被鉴定为交叉污染。在后一组中，发现PC-93是HeLa的衍生物，而DuPro-1、ALVA-55和ALVA-101是PC-3的衍生物。17种真正的前列腺细胞系表达角蛋白8（K8）和K18。九种细胞系显示AR表达，其中五种在AR基因中存在突变。仅在表达AR的细胞系中检测到前列腺特异性抗原和DD3。七种细胞系表达基底细胞标志物K5，其中三种细胞系显示AR共表达。
本研究定义了一组17种真正的前列腺癌细胞系。该细胞系集合虽然规模较小，但构成了前列腺癌的各种不同类型和阶段，同时也部分反映了这种恶性肿瘤的异质性。
