Lau Aik-Jiang, Woo Soo-On, Koh Hwee-Ling
Department of Pharmacy, Faculty of Science, National University of Singapore, 18 Science Drive 4, Singapore 117543, Singapore.
J Chromatogr A. 2003 Sep 5;1011(1-2):77-87. doi: 10.1016/s0021-9673(03)01135-x.
A reversed-phase high-performance liquid chromatography-diode array detection method was developed and validated for the simultaneous determination of six saponins (notoginsenoside R1, ginsenosides Rg1, Re, Rb1, Rc, Rd) in raw and steamed Panax notoginseng. Linearity (r2 > 0.9988), intra- and inter-day precision (RSD < 4%), limit of detection (0.008-0.013 mg/ml), limit of quantification (0.027-0.042 mg/ml) of the saponins were determined. The method was successfully applied to 11 pairs of raw and steamed P. notoginseng products. Three products showed discrepancies between theirlabelled claims (raw or steamed) and the results of analysis. This new, simple and reliable method could be used in the quality control of raw and steamed P. notoginseng.
建立了一种反相高效液相色谱 - 二极管阵列检测方法，并进行了验证，用于同时测定生三七和蒸制三七中的六种皂苷（三七皂苷R1、人参皂苷Rg1、Re、Rb1、Rc、Rd）。测定了皂苷的线性关系（r2 > 0.9988）、日内和日间精密度（RSD < 4%）、检测限（0.008 - 0.013 mg/ml）和定量限（0.027 - 0.042 mg/ml）。该方法成功应用于11对生三七和蒸制三七产品。三种产品的标签声明（生或蒸制）与分析结果存在差异。这种新的、简单可靠的方法可用于生三七和蒸制三七的质量控制。
