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谷胱甘肽通过1型兰尼碱受体通道转运的证据。

Evidence for the transport of glutathione through ryanodine receptor channel type 1.

作者信息

Bánhegyi Gábor, Csala Miklós, Nagy Gábor, Sorrentino Vincenzo, Fulceri Rosella, Benedetti Angelo

机构信息

Orvosi Vegytani, Molekuláris Biológiai és Pathobiokémiai Intézet, Semmelweis Egyetem, Budapest, Hungary.

出版信息

Biochem J. 2003 Dec 15;376(Pt 3):807-12. doi: 10.1042/BJ20031419.

DOI:10.1042/BJ20031419
PMID:14519101
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1223822/
Abstract

In the present study, we have investigated the role of RyR1 (ryanodine receptor calcium channel type 1) in glutathione (GSH) transport through the sarcoplasmic reticulum (SR) membrane of skeletal muscles. Lanthanum chloride, a prototypic blocker of cation channels, inhibited the influx and efflux of GSH in SR vesicles. Using a rapid-filtration-based assay and lanthanum chloride as a transport blocker, an uptake of radiolabelled GSH into SR vesicles was observed. Pretreatment of SR vesicles with the RyR1 antagonists Ruthenium Red and ryanodine as well as with lanthanum chloride blocked the GSH uptake. An SR-like GSH uptake appeared in microsomes obtained from an HEK-293 (human embryonic kidney 293) cell line after transfection of RyR1. These observations strongly suggest that RyR1 mediates GSH transport through the SR membranes of skeletal muscles.

摘要

在本研究中，我们研究了1型兰尼碱受体钙通道（RyR1）在谷胱甘肽（GSH）通过骨骼肌肌浆网（SR）膜转运中的作用。阳离子通道的典型阻滞剂氯化镧抑制了GSH在SR囊泡中的流入和流出。使用基于快速过滤的测定法并以氯化镧作为转运阻滞剂，观察到放射性标记的GSH被摄取到SR囊泡中。用RyR1拮抗剂钌红和兰尼碱以及氯化镧预处理SR囊泡可阻断GSH的摄取。转染RyR1后，从人胚肾293（HEK-293）细胞系获得的微粒体中出现了类似SR的GSH摄取。这些观察结果强烈表明，RyR1介导GSH通过骨骼肌的SR膜进行转运。

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