Turell Michael J, Bunning Michel, Ludwig George V, Ortman Brian, Chang Jeff, Speaker Tully, Spielman Andrew, McLean Robert, Komar Nicholas, Gates Robert, McNamara Tracey, Creekmore Terry, Farley Linda, Mitchell Carl J
Department of Vector Assessment, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland 21702-5011, USA.
Emerg Infect Dis. 2003 Sep;9(9):1077-81. doi: 10.3201/eid0909.030025.
A DNA vaccine for West Nile virus (WNV) was evaluated to determine whether its use could protect fish crows (Corvus ossifragus) from fatal WNV infection. Captured adult crows were given 0.5 mg of the DNA vaccine either orally or by intramuscular (IM) inoculation; control crows were inoculated or orally exposed to a placebo. After 6 weeks, crows were challenged subcutaneously with 105 plaque-forming units of WNV (New York 1999 strain). None of the placebo inoculated-placebo challenged birds died. While none of the 9 IM vaccine-inoculated birds died, 5 of 10 placebo-inoculated and 4 of 8 orally vaccinated birds died within 15 days after challenge. Peak viremia titers in birds with fatal WNV infection were substantially higher than those in birds that survived infection. Although oral administration of a single DNA vaccine dose failed to elicit an immune response or protect crows from WNV infection, IM administration of a single dose prevented death and was associated with reduced viremia.
对一种针对西尼罗河病毒（WNV）的DNA疫苗进行了评估，以确定其使用是否能保护鱼鸦（Corvus ossifragus）免受致命的WNV感染。捕获的成年乌鸦通过口服或肌肉注射（IM）接种0.5毫克DNA疫苗；对照乌鸦接种或口服安慰剂。6周后，乌鸦皮下接种105个空斑形成单位的WNV（1999年纽约毒株）进行攻毒。接种安慰剂-攻毒安慰剂的鸟类均未死亡。虽然9只肌肉注射疫苗的鸟类均未死亡，但10只接种安慰剂和8只口服疫苗的鸟类中有5只和4只在攻毒后15天内死亡。发生致命WNV感染的鸟类的病毒血症峰值滴度显著高于存活感染鸟类。虽然口服单剂量DNA疫苗未能引发免疫反应或保护乌鸦免受WNV感染，但肌肉注射单剂量可预防死亡并降低病毒血症。
