Tang Ning, Zhu Jin Xia, Zhao Wen Chao, Xing Ying, Gou Yu Lin, Rowlands Dewi Kenneth, Chung Yiu Wa, Chan Hsiao Chang
Department of Physiology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong.
Biol Pharm Bull. 2003 Oct;26(10):1384-7. doi: 10.1248/bpb.26.1384.
We have recently demonstrated that Bak Foong Pills (BFP), a well-known Chinese medicine widely used for treating gynecological disorders, stimulates human colonic epithelial anion secretion, which was mediated by intracellular cAMP and Ca(2+). The present study further investigated the effect of BFP on exocrine pancreatic-bile secretion using in vivo and in vitro approaches. Duodenal infusion of BFP ethanol extract (1 g/kg) in rats produced increases in the volume and protein output of pancreatic-bile juice, but did not affect its pH. Surgical ablation of vagal neural pathway slightly reduced the effect of BFP on the protein output and volume, indicating that the vagal nerve pathway was not the major player in medicating the effect of BFP on exocrine pancreatic-bile secretion. Using CAPAN-1 cell line, a human pancreatic duct cell line, in conjunction with the short-circuit current (I(SC)) measurements, we further demonstrated that BFP could directly stimulate pancreatic HCO(3)(-) secretion. Basolateral addition of BFP (600 microg/ml) produced averaged charges transported of 2100+/-382.5 microC/cm(2), which was blocked by apical addition of Cl(-) channel blocker. Removal of HCO(3)(-) from the Krebs-Henseleit (K-H) solution inhibited the BFP-induced I(SC) by more than 95%. The present results suggest that BFP could improve digestive function by stimulating pancreatic protein and HCO(3)(-) secretion.
我们最近证明，白凤丸（BFP），一种广泛用于治疗妇科疾病的著名中药，可刺激人结肠上皮细胞阴离子分泌，该过程由细胞内cAMP和Ca（2+）介导。本研究使用体内和体外方法进一步研究了BFP对外分泌性胰胆汁分泌的影响。给大鼠十二指肠输注BFP乙醇提取物（1 g/kg）可使胰胆汁的体积和蛋白质输出量增加，但不影响其pH值。迷走神经通路的手术切除略微降低了BFP对蛋白质输出量和体积的影响，这表明迷走神经通路不是介导BFP对外分泌性胰胆汁分泌作用的主要因素。使用人胰腺导管细胞系CAPAN-1细胞系并结合短路电流（I（SC））测量，我们进一步证明BFP可以直接刺激胰腺HCO（3）（-）分泌。在基底外侧添加BFP（600 microg/ml）产生的平均电荷转运为2100+/-382.5 microC/cm（2），这被在顶端添加Cl（-）通道阻滞剂所阻断。从Krebs-Henseleit（K-H）溶液中去除HCO（3）（-）可使BFP诱导的I（SC）抑制超过95%。目前的结果表明，BFP可以通过刺激胰腺蛋白质和HCO（3）（-）分泌来改善消化功能。
