用于香菇转化的同源选择标记基因的构建。
Construction of a homologous selectable marker gene for Lentinula edodes transformation.
作者信息
Irie Toshikazu, Sato Toshitsugu, Saito Kumiko, Honda Yoichi, Watanabe Takashi, Kuwahara Masaaki, Enei Hitoshi
机构信息
Iwate Biotechnology Research Center, Kitakami, Iwate, Japan.
出版信息
Biosci Biotechnol Biochem. 2003 Sep;67(9):2006-9. doi: 10.1271/bbb.67.2006.
PMID:14519992
Abstract
We cloned a gene for the iron sulfur protein (Ip) subunit from an edible mushroom, Lentinula edodes, and introduced a point mutation that confers carboxin resistance into it. The mutant gene successfully transformed L. edodes with high efficiency (9 transformants/2.5 microg vector DNA). Restriction enzyme-mediated integration (REMI) increased the transformation efficiency by about two-fold.
摘要
我们从食用菌香菇中克隆了铁硫蛋白（Ip）亚基的基因，并在其中引入了赋予萎锈灵抗性的点突变。突变基因成功地高效转化了香菇（每2.5微克载体DNA有9个转化体）。限制酶介导整合（REMI）使转化效率提高了约两倍。
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