Irie Toshikazu, Sato Toshitsugu, Saito Kumiko, Honda Yoichi, Watanabe Takashi, Kuwahara Masaaki, Enei Hitoshi
Iwate Biotechnology Research Center, Kitakami, Iwate, Japan.
Biosci Biotechnol Biochem. 2003 Sep;67(9):2006-9. doi: 10.1271/bbb.67.2006.
We cloned a gene for the iron sulfur protein (Ip) subunit from an edible mushroom, Lentinula edodes, and introduced a point mutation that confers carboxin resistance into it. The mutant gene successfully transformed L. edodes with high efficiency (9 transformants/2.5 microg vector DNA). Restriction enzyme-mediated integration (REMI) increased the transformation efficiency by about two-fold.
我们从食用菌香菇中克隆了铁硫蛋白（Ip）亚基的基因，并在其中引入了赋予萎锈灵抗性的点突变。突变基因成功地高效转化了香菇（每2.5微克载体DNA有9个转化体）。限制酶介导整合（REMI）使转化效率提高了约两倍。
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