利用几丁质合成酶基因启动子构建香菇转化的新型载体。
Construction of novel vectors for transformation of Lentinula edodes using a chitin synthase gene promoter.
机构信息
Iwate Biotechnology Research Center, 22-174-4 Narita, Kitakami, Iwate 024-0003, Japan.
出版信息
J Biosci Bioeng. 2011 Feb;111(2):117-20. doi: 10.1016/j.jbiosc.2010.10.003. Epub 2010 Nov 1.
PMID:21044863
Abstract
Novel vectors (pLCHS-hph and pChG-bar) containing expression unit driving hph as a selectable marker gene by chitin synthase gene promoter were constructed for Lentinula edodes transformation. Expression of the hph gene in random selected transformants was confirmed by RT-PCR method. Thus, both vectors are useful for L. edodes transformation.
摘要
构建了含有几丁质合成酶基因启动子驱动潮霉素磷酸转移酶（hph）作为选择标记基因的表达单元的新型载体（pLCHS-hph 和 pChG-bar），用于香菇转化。通过 RT-PCR 方法证实了随机选择的转化子中 hph 基因的表达。因此，这两个载体都可用于香菇转化。
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