Takashiba S, Takigawa M, Takahashi K, Myokai F, Nishimura F, Chihara T, Kurihara H, Nomura Y, Murayama Y
Department of Periodontology and Endodontology, Okayama University Dental School, Japan.
Infect Immun. 1992 Dec;60(12):5253-8. doi: 10.1128/iai.60.12.5253-5258.1992.
Inflammatory mediators produced by cells in the gingiva have been implicated in the initiation and progression of periodontal disease, a common infectious disease. In this study, we examined the biological activity of neutrophil chemotactic factors and the kinetics of expression of interleukin-8 (IL-8) mRNA derived from normal gingival fibroblasts in response to inflammatory mediators in an in vitro model. Gingival fibroblasts stimulated by either recombinant human interleukin-1 beta or recombinant human tumor necrosis factor alpha produced neutrophil chemotactic factors after 4 h, whereas expression of cell-derived IL-8 mRNA was detected within 1 h after stimulation. Furthermore, in a neutralization assay, rabbit anti-recombinant human IL-8 antiserum inhibited neutrophil chemotactic activity to basal levels. These results provide evidence that gingival fibroblasts synthesize potent chemotactic factors such as IL-8 in the presence of the inflammatory mediators interleukin-1 beta and tumor necrosis factor alpha. The activity of these factors may contribute to neutrophil-mediated processes in the pathogenesis of periodontal disease.
牙龈中的细胞产生的炎症介质与牙周病（一种常见的传染病）的发生和发展有关。在本研究中，我们在体外模型中检测了中性粒细胞趋化因子的生物活性以及正常牙龈成纤维细胞衍生的白细胞介素-8（IL-8）mRNA对炎症介质反应的表达动力学。用重组人白细胞介素-1β或重组人肿瘤坏死因子α刺激牙龈成纤维细胞4小时后产生中性粒细胞趋化因子，而在刺激后1小时内检测到细胞衍生的IL-8 mRNA的表达。此外，在中和试验中，兔抗重组人IL-8抗血清将中性粒细胞趋化活性抑制至基础水平。这些结果提供了证据，表明牙龈成纤维细胞在炎症介质白细胞介素-1β和肿瘤坏死因子α存在的情况下合成强效趋化因子，如IL-8。这些因子的活性可能有助于牙周病发病机制中中性粒细胞介导的过程。
