Gao Ju, Zeng Bangxiong, Zhou Luojing, Yuan Shiying, Zhang Shihai
Department of Anesthesiology, Xiehe Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022.
J Huazhong Univ Sci Technolog Med Sci. 2003;23(3):320-3. doi: 10.1007/BF02829527.
To assess the potential therapeutic effect of propofol in the treatment of endotoxemia, 76 rats were randomly assigned to 5 groups: control group(A), endotoxemic group(B), pre-treatment group(C), simultaneous treatment group(D) and post-treatment group(E). Five h after endotoxin injection, PO2, pH, MAP, plasma concentrations of Nitrite/nitrate (NO2-/NO3-) and mortality rates were assessed in each group. After the rats were sacrificed, lung tissue was sampled to measure myeloperoxidase (MPO) activity and tumor necrosis factor (TNF)-alpha contents. It was found that endotoxin injection produced progressive hypotension, metabolic acidosis, and a large increase in the plasma NO2-/NO3- concentrations and increased mortality rates in 5 h. Endotoxin injection significantly increased MPO activity and TNF-alpha contents in lung tissue (P < 0.01 or P < 0.05). These changes response to endotoxin were significantly attenuated in the groups B, C and D. But these beneficial effects were blunted in the group E. The results suggest that propofol administration may offer advantages in endotoxemia.
为评估丙泊酚对内毒素血症的潜在治疗效果，将76只大鼠随机分为5组：对照组（A）、内毒素血症组（B）、预处理组（C）、同时治疗组（D）和后处理组（E）。内毒素注射后5小时，评估每组的动脉血氧分压（PO2）、酸碱度（pH）、平均动脉压（MAP）、血浆亚硝酸盐/硝酸盐（NO2-/NO3-）浓度及死亡率。大鼠处死后，采集肺组织样本检测髓过氧化物酶（MPO）活性和肿瘤坏死因子（TNF）-α含量。结果发现，内毒素注射导致5小时内出现进行性低血压、代谢性酸中毒，血浆NO2-/NO3-浓度大幅升高及死亡率增加。内毒素注射显著增加肺组织中MPO活性和TNF-α含量（P<0.01或P<0.05）。这些对内毒素的反应变化在B、C和D组中明显减弱。但这些有益作用在E组中减弱。结果表明，给予丙泊酚可能对内毒素血症有益。
