Alpas Hami, Lee Jaesung, Bozoglu Faruk, Kaletunç Gönül
Food Engineering Department, Middle East Technical University, 06531 Ankara, Turkey.
Int J Food Microbiol. 2003 Nov 1;87(3):229-37. doi: 10.1016/s0168-1605(03)00066-7.
Differential scanning calorimetry (DSC) was used to evaluate the relative high hydrostatic pressure (HHP) resistances of bacterial strains from Staphylococcus aureus and Escherichia coli O157:H7 in vivo. The total apparent enthalpy change and thermal stability were two DSC parameters used to compare bacterial strains of untreated control and pressure-treated bacteria. DSC thermograms indicated that ribosomal denaturation appears to be a major factor in cell death by both thermal and high pressure treatments. However, the analysis of calorimetric data for control samples as well as pressure-treated samples clearly showed that the sensitivities of bacteria to various physical stresses can be different. While S. aureus 765 had a relatively higher resistance to thermal treatment in comparison to S. aureus 485, S. aureus 485 was determined to be more resistant to pressure than S. aureus 765. This information can be utilized in the design of processes specific to targeting certain cellular components by using different physical stresses.
差示扫描量热法（DSC）用于评估体内金黄色葡萄球菌和大肠杆菌O157:H7菌株对相对高静水压（HHP）的抗性。总表观焓变和热稳定性是用于比较未处理对照细菌菌株和压力处理细菌菌株的两个DSC参数。DSC热分析图表明，核糖体变性似乎是热处理和高压处理导致细胞死亡的主要因素。然而，对对照样品以及压力处理样品的量热数据进行分析清楚地表明，细菌对各种物理应激的敏感性可能不同。与金黄色葡萄球菌485相比，金黄色葡萄球菌765对热处理具有相对较高的抗性，而金黄色葡萄球菌485被确定对压力的抗性比金黄色葡萄球菌765更强。这些信息可用于设计特定的工艺，通过使用不同的物理应激来靶向某些细胞成分。
