Hu Min, Sabelman Eric E, Cao Yang, Chang James, Hentz Vincent R
Functional Restoration Department, Stanford University School of Medicine, CA, USA.
J Biomed Mater Res B Appl Biomater. 2003 Oct 15;67(1):586-92. doi: 10.1002/jbm.b.20001.
Hyaluronic acid (HA) has been found to play important roles in tissue regeneration and wound-healing processes. Fetal tissue with a high concentration of HA heals rapidly without scarring. The present study employed HA formed into three-dimensional strands with or without keratinocytes to treat full-thickness skin incision wounds in rats. Wound closure rates of HA strand grafts both with and without keratinocytes were substantially enhanced. The closure times of both HA grafts were less than 1 day (average 16 h), about 1/7 that of the contralateral control incisions (114 h, p <.01). Average wound areas after 10 days were HA-only graft: 0.151 mm2 +/- 0.035; HA + cell grafts: 0.143 mm2 +/- 0.036 and controls: 14.434 mm2 +/- 1.175, experimental areas were 1% of the controls (p < 0.01). Transforming growth factor (TGF) beta1 measured by immunostaining was remarkably reduced in HA-treated wounds compared to the controls. In conclusion, HA grafts appeared to produce a fetal-like environment with reduced TGF-beta1, which is known to be elevated in incipient scars. The HA strands with or without cultured cells may potentially improve clinical wound healing as well as reduce scar formation.
透明质酸（HA）已被发现可在组织再生和伤口愈合过程中发挥重要作用。含有高浓度HA的胎儿组织愈合迅速且不留疤痕。本研究采用含或不含角质形成细胞的三维HA丝来治疗大鼠的全层皮肤切口伤口。含角质形成细胞和不含角质形成细胞的HA丝移植物的伤口闭合率均显著提高。两种HA移植物的闭合时间均小于1天（平均16小时），约为对侧对照切口（114小时，p <.01）的1/7。10天后的平均伤口面积分别为：仅HA移植物：0.151平方毫米±0.035；HA + 细胞移植物：0.143平方毫米±0.036；对照组：14.434平方毫米±1.175，实验组面积为对照组的1%（p < 0.01）。通过免疫染色测量发现，与对照组相比，HA处理的伤口中转化生长因子（TGF）β1显著降低。总之，HA移植物似乎营造了一种类似胎儿的环境，其中TGF-β1含量降低，而TGF-β1在初期疤痕中含量升高是已知的。含或不含培养细胞的HA丝可能会改善临床伤口愈合并减少疤痕形成。
