柴胡总皂苷抗内毒素作用的研究

[Studies on antiendotoxin action of total saponins from radix Bupleuri].

作者信息

Liu Yunhai, Chen Yongshun, Xi Wei, Bai Jie

机构信息

Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030.

出版信息

Zhong Yao Cai. 2003 Jun;26(6):423-5.

PMID:14528684

Abstract

OBJECTIVE

To study antiendotoxin activities of total saponins from Radix Bupleuri.

METHOD

The antiendotoxin activities were demonstrated by Limulus Amebocyte Lysate test (LAL test) in vitro, the fever model induced by endotoxin in rabbits and the model induced by LPS in BCG-primed mice.

RESULTS

Total saponins from Radix Bupleuri still had antiendotoxin effect even when it (50 mg.ml-1) was diluted to 32 times by LAL test in vitro. The fever experiment results showed the average rising temperature of BCS + LPS, group, model group and BCS group was 0.75 degrees C, -0.25 degrees C and -0.37 degrees C respectively. In the experiment of the poison induced by LPS, fatal rate of model group was 90.0%, that of BCS + LPS group was 13.3%, there was a significant difference (P < 0.01).

CONCLUSION

The result showed that the total saponins in Bupleuri had the strongest antiendotoxin action.

摘要

目的

研究柴胡总皂苷的抗内毒素活性。

方法

采用鲎试剂法（LAL 法）体外检测其抗内毒素活性，通过家兔内毒素致热模型及卡介苗致敏小鼠 LPS 致毒模型进行验证。

结果

体外鲎试剂法检测中，柴胡总皂苷（50mg·ml-1）即使稀释 32 倍仍具有抗内毒素作用。发热实验结果显示，BCS+LPS 组、模型组和 BCS 组的平均升温分别为 0.75℃、-0.25℃和-0.37℃。在 LPS 致毒实验中，模型组的死亡率为 90.0%，BCS+LPS 组为 13.3%，差异有统计学意义（P<0.01）。