Koekemoer L L, Rankoe E M, la Grange J P, Govere J, Coetzee M
Medical Entomology, Department of Clinical Microbiology and Infectious Diseases, School of Pathology of the South African Institute for Medical Research, University of the Witwatersrand, PO Box 1038, Johannesburg, 2000, South Africa.
J Am Mosq Control Assoc. 2001 Sep;17(3):160-5.
A study was conducted to determine the role of members of the Anopheles funestus group in malaria transmission in the Mpumalanga Province, in the northeastern region of South Africa. Female anopheline mosquitoes were collected between January 1996 and November 1997 by means of human landing catches and tested for salivary gland Plasmodium falciparum infections by means of the enzyme-linked immunosorbent assay (ELISA) method with PF2A10 antibodies. Infection rates from April and May 1997 collections were 3.73% and 19.4%, respectively. None of the nonimmune collectors became infected with malaria. The ELISA-positive mosquitoes were tested with a polymerase chain reaction (PCR) malaria detection assay based on sequence variation present in the small subunit ribosomal RNA gene. Only 1.09% of ELISA-positive mosquitoes were PCR-positive for malaria. Initially, all mosquitoes were assumed to belong to the An. funestus group but subsequent molecular taxonomy showed this assumption to be false. The use of a single-strand conformation polymorphism (SSCP) assay revealed only 1 member of the An. funestus group, An. rivulorum. All other specimens produced banding patterns not seen before. Those samples were identified morphologically as An. demeilloni and An. marshallii s.l. These 2 species are not recognized malaria vectors and thus it is possible that the ELISA results are misleading.
开展了一项研究，以确定南非东北部姆普马兰加省的嗜人按蚊种群成员在疟疾传播中的作用。1996年1月至1997年11月期间，通过人饵诱捕法收集雌性按蚊，并采用酶联免疫吸附测定（ELISA）法，使用PF2A10抗体检测唾液腺恶性疟原虫感染情况。1997年4月和5月收集的样本感染率分别为3.73%和19.4%。所有非免疫采集者均未感染疟疾。对ELISA呈阳性的蚊子进行基于小亚基核糖体RNA基因序列变异的聚合酶链反应（PCR）疟疾检测分析。只有1.09%的ELISA阳性蚊子经PCR检测为疟疾阳性。最初，所有蚊子都被假定属于嗜人按蚊种群，但随后的分子分类学表明这一假设是错误的。单链构象多态性（SSCP）分析显示，嗜人按蚊种群中只有一个成员，即溪流按蚊。所有其他样本产生了以前未见的条带模式。这些样本经形态学鉴定为德氏按蚊和马氏按蚊复合组。这两个物种不被认为是疟疾传播媒介，因此ELISA结果可能具有误导性。
