Temu E A, Minjas J N, Tuno N, Kawada H, Takagi M
Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan.
Acta Trop. 2007 May;102(2):119-25. doi: 10.1016/j.actatropica.2007.04.009. Epub 2007 Apr 22.
The role of Anopheles funestus group in malaria transmission was investigated in Bagamoyo coastal Tanzania, in the process of characterizing the area as a malaria vaccine testing site. Mosquitoes were sampled inside houses and multiplex PCR was used to identify 649 specimens. The following species were found: A. funestus s.s. (84.3%), A. leesoni (13.6%), A. rivulorum (1.5%) and A. parensis (0.6%). Multiplex PCR of 147 blood-fed specimens showed that over half (57.1%) of the identifiable blood meals were taken from human hosts, and human blood index in A. funestus and A. leesoni was 55% and 82% respectively. Plasmodium falciparum infection rate determined by nested PCR was 11% in A. funestus s.s. Although the abundance was low, 26 specimens of A. leesoni, two of A. rivolurum and one of A. parensis were found positive for P. falciparum. The presence of four A. funestus species in Tanzania emphasizes the relevance to define precisely their spatial and temporal distribution, specific behaviour, ecology and exact role in malaria transmission.
在坦桑尼亚巴加莫约沿海地区将该区域确定为疟疾疫苗测试地点的过程中，对费氏按蚊种群在疟疾传播中的作用进行了调查。在房屋内采集蚊子样本，并使用多重PCR对649个样本进行鉴定。发现了以下物种：费氏按蚊指名亚种（84.3%）、李氏按蚊（13.6%）、溪流按蚊（1.5%）和帕氏按蚊（0.6%）。对147个吸食血液的样本进行多重PCR检测显示，超过一半（57.1%）可识别的血餐来自人类宿主，费氏按蚊和李氏按蚊的人类血液指数分别为55%和82%。通过巢式PCR测定，费氏按蚊指名亚种的恶性疟原虫感染率为11%。尽管数量较少，但发现26个李氏按蚊样本、2个溪流按蚊样本和1个帕氏按蚊样本的恶性疟原虫呈阳性。坦桑尼亚存在四种费氏按蚊，这凸显了精确界定它们的时空分布、特定行为、生态以及在疟疾传播中的确切作用的重要性。
