Shen Ben, Liu Wen, Nonaka Koichi
Division of Pharmaceutical Sciences and Department of Chemistry, University of Wisconsin, Madison, WI 53705, USA.
Curr Med Chem. 2003 Nov;10(21):2317-25. doi: 10.2174/0929867033456701.
This review gives a brief account on the current status of enediyne biosynthesis and the prospective of applying combinatorial biosynthesis methods to the enediyne system for novel analog production. Methods for cloning enediyne biosynthetic gene clusters are first reviewed. A unified paradigm for enediyne biosynthesis, characterized with (a) the enediyne PKS, (b) the enediyne PKS accessory enzymes, and (c) tailoring enzymes, is then presented. Strategies and tools for novel enediyne analog production by combinatorial biosynthesis are finally discussed. The results set the stage to decipher the molecular mechanism for enediyne biosynthesis and lay the foundation to engineer novel enediynes by combinatorial biosynthesis for future endeavors.
本综述简要介绍了烯二炔生物合成的现状以及将组合生物合成方法应用于烯二炔系统以生产新型类似物的前景。首先综述了克隆烯二炔生物合成基因簇的方法。然后提出了烯二炔生物合成的统一模式，其特征为（a）烯二炔聚酮合酶，（b）烯二炔聚酮合酶辅助酶，以及（c）修饰酶。最后讨论了通过组合生物合成生产新型烯二炔类似物的策略和工具。这些结果为解析烯二炔生物合成的分子机制奠定了基础，并为未来通过组合生物合成工程改造新型烯二炔奠定了基础。
