Brenwald N P, Fraise A P
Department of Microbiology, City Hospital, Dudley Road, Birmingham B18 7QH, UK.
J Hosp Infect. 2003 Oct;55(2):141-4. doi: 10.1016/s0195-6701(03)00222-6.
Two triclosan selected mutants showed four-fold and 16-fold increases in their minimum inhibitory concentrations (MICs) of triclosan (1 mg/L and 4 mg/L) compared with their parent strains. Four clinical isolates of MRSA were detected with the same triclosan susceptibility as the mutants. One mutant had a predicted change in the gene product on FabI (Thr 147-->His), whilst only one clinical isolate had predicted FabI amino-acid changes (Ala 198-->Gly, and Leu 208-->Phe). The lack of fabI mutations in one mutant and three of the clinical isolates showing reduced triclosan susceptibility suggest that genetic loci other than fabI may be involved in triclosan resistance.
与亲本菌株相比，两种经三氯生筛选的突变体对三氯生（1毫克/升和4毫克/升）的最低抑菌浓度（MIC）分别增加了四倍和十六倍。检测到四株耐甲氧西林金黄色葡萄球菌临床分离株对三氯生的敏感性与这些突变体相同。一个突变体的FabI基因产物有预测的变化（苏氨酸147→组氨酸），而只有一株临床分离株有预测的FabI氨基酸变化（丙氨酸198→甘氨酸，亮氨酸208→苯丙氨酸）。一个突变体和三株对三氯生敏感性降低的临床分离株中缺乏fabI突变，这表明除fabI外的其他基因位点可能与三氯生耐药性有关。
