Gonzalez J M, Ortiz-Martinez A, Gonzalez-delValle M A, Laiz L, Saiz-Jimenez C
Instituto de Recursos Naturales y Agrobiologia, Consejo Superior de Investigaciones Científicas, P.O. Box 1052, 41080, Seville, Spain.
J Microbiol Methods. 2003 Nov;55(2):459-63. doi: 10.1016/s0167-7012(03)00171-4.
We propose a strategy for the efficient screening of large libraries of amplified 16S rRNA genes from complex environmental samples. It consists of processing sets of multiple clones simultaneously. This strategy saves up to 90% of the costs and labor spent in the process of screening a 16S rDNA library.
我们提出了一种从复杂环境样本中高效筛选大量扩增16S rRNA基因文库的策略。该策略包括同时处理多个克隆集。此策略可节省筛选16S rDNA文库过程中高达90%的成本和劳动力。
