通过红外常压基质辅助激光解吸电离对非共价糖-糖复合物进行碎片化研究。

Fragmentation studies of noncovalent sugar-sugar complexes by infrared atmospheric pressure MALDI.

作者信息

Von Seggern Christopher E, Cotter Robert J

机构信息

Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, 725 N. Wolfe Street, B-7 Biophysics Building, Baltimore, MD, USA.

出版信息

J Am Soc Mass Spectrom. 2003 Oct;14(10):1158-65. doi: 10.1016/S1044-0305(03)00478-1.

DOI:10.1016/S1044-0305(03)00478-1

PMID:14530096

Abstract

An investigation of sugar-sugar noncovalent complex fragmentation was conducted using a 2.94 microm Er:YAG laser for infrared (IR) atmospheric pressure matrix-assisted laser desorption/ionization (AP MALDI) on an ion trap mass spectrometer (ITMS). This approach allowed the analysis of weak noncovalent complexes between a variety of biologically relevant oligosaccharides. The strength of interaction varied with different sugar structures, potentially due to varying strength of hydrogen bonding networks. In some cases, fragmentation of intramolecular sugar bonds preceded breakdown of the noncovalent complex. This result appeared primarily when complexes contained sugars with at least one sialic acid. Globotrios dimers also showed intramolecular fragmentation in preference to breakdown of the noncovalent dimer. This technique will allow further study of sugar-sugar interactions known to play a role in cellular interactions.

摘要

使用波长为2.94微米的铒：钇铝石榴石（Er:YAG）激光，在离子阱质谱仪（ITMS）上进行红外（IR）常压基质辅助激光解吸/电离（AP MALDI），对糖-糖非共价复合物的碎裂进行了研究。这种方法能够分析多种具有生物学相关性的寡糖之间的弱非共价复合物。相互作用的强度因糖结构的不同而有所变化，这可能是由于氢键网络强度的差异所致。在某些情况下，分子内糖键的断裂先于非共价复合物的分解。这一结果主要出现在复合物中含有至少一个唾液酸的糖类时。二聚体葡萄糖三糖也表现出分子内断裂优先于非共价二聚体的分解。这项技术将有助于进一步研究已知在细胞相互作用中发挥作用的糖-糖相互作用。