Das Gopal, Talukdar Pinaki, Matile Stefan
Department of Organic Chemistry, University of Geneva, CH-1211 Geneva, Switzerland.
Science. 2002 Nov 22;298(5598):1600-2. doi: 10.1126/science.1077353.
The reversible blockage of synthetic pores formed by rigid-rod beta barrels, either by substrates or products, was used to sense a variety of enzymatic reactions in high-throughput format with "naked-eye" fluorescent detection. Improvement of sensor sensitivity beyond three orders of magnitude by straightforward internal mutations underscores the functional plasticity of rigid-rod beta barrels. Such detectors of enzyme activity with the aforementioned characteristics are needed in areas as diverse as proteomics and environmentally benign organic synthesis.
由刚性杆状β桶形成的合成孔的可逆阻塞，无论是被底物还是产物阻塞，都被用于通过“肉眼”荧光检测以高通量形式检测各种酶促反应。通过直接的内部突变将传感器灵敏度提高三个数量级以上，突出了刚性杆状β桶的功能可塑性。蛋白质组学和环境友好型有机合成等不同领域都需要具有上述特性的酶活性检测器。
