Schäfer A, Pauli G, Friedmann W, Dudenhausen J W
Department of Obstetrics and Gynecology, Rudolf Virchow Hospital, Free University Berlin, Fed. Rep. of Germany.
J Perinat Med. 1992;20(3):233-40. doi: 10.1515/jpme.1992.20.3.233.
The effect of the human trophoblast hormones chorionic gonadotropin (hCG) and placental lactogen (hPL) on the expression of cytokines in peripheral blood mononuclear cell cultures was followed under a variety of culture conditions, (a) phytohemagglutinin stimulated cells (PHA-MSC), (b) allogenic mixed cells (AMC) and (c) spontaneously proliferating cells (SPC). A dose dependent enhanced release of IL-6, IL-1 beta and TNF-alpha by hCG and hPL was observed under all culture conditions. However, an inhibitory effect on the IL-2 release was seen in PHA-MSC by hPL and in AMC by hPL and hCG. The role of the suppression of IL-2 production/release on cytotoxicity towards trophoblast is discussed. These results suggest a sensitive, dose dependent hormonal control of the modulation of the immune response during pregnancy and strengthen the concept of a distinct regulation of monocytes and lymphocyte subpopulation by trophoblast hormones.
在多种培养条件下，研究了人滋养层激素绒毛膜促性腺激素（hCG）和胎盘催乳素（hPL）对外周血单个核细胞培养物中细胞因子表达的影响，这些条件包括：（a）植物血凝素刺激的细胞（PHA-MSC）、（b）同种异体混合细胞（AMC）和（c）自发增殖细胞（SPC）。在所有培养条件下，均观察到hCG和hPL呈剂量依赖性地增强IL-6、IL-1β和TNF-α的释放。然而，hPL对PHA-MSC中的IL-2释放有抑制作用，hPL和hCG对AMC中的IL-2释放也有抑制作用。讨论了抑制IL-2产生/释放对滋养层细胞毒性的作用。这些结果表明，孕期免疫反应调节存在敏感的、剂量依赖性的激素控制，强化了滋养层激素对单核细胞和淋巴细胞亚群有独特调节作用的概念。
