Fang Wen, Bao Huai-En, Xiao Liang-Liang, Mu Rong
Department of Clinical Laboratory Sciences, Guiyang Medical College, Guiyang, China.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2009 Aug;27(4):313-7.
To screen and identify specific antigens of Taenia solium cysticercus, and predict the function of target proteins using bioinformatics method.
Patients infected with Taenia solium were dewormed by decoction arecae and pumpkin seeds to collect worms, and eggs were then prepared. Six three-way crossed hybrid pigs were randomly divided into experimental group and control group, and each experimental pig was infected with 80,000 T. solium eggs. Serum samples were collected at 40 days after infection. The total protein of T. solium cysticercus was separated by two-dimensional electrophoresis, and Western blotting was performed to find out distinct antigens. Proteins from the two groups were identified by ESI-Trap MS. Query in NCBI database was made to confirm function of the proteins.
207+/-9 spots were detected through Coomassie brilliant blue-stained gels with Mr 14,400-94,000 and pI 3.0-10.0. Western blotting showed 7 specific antigen spots with pool sera of infected pigs. Four of the 7 antigens with known functions were respectively ascribed to cytoskeletal actin-2 (adult-specific), tropomyosin (cysticercus-specific), AF239799-1 annexin (cysticercus-specific) and actin-1 (cysticercus-specific).
Three specific antigens of Taenia solium cysticercus have been identified.
筛选并鉴定猪带绦虫囊尾蚴的特异性抗原,并用生物信息学方法预测靶蛋白的功能。
用槟榔和南瓜子煎剂对猪带绦虫感染患者进行驱虫以收集虫体,然后制备虫卵。将6头三元杂交猪随机分为实验组和对照组,每头实验猪感染80000个猪带绦虫卵。感染后40天采集血清样本。采用双向电泳分离猪带绦虫囊尾蚴的总蛋白,进行Western印迹分析以找出不同的抗原。通过电喷雾离子阱质谱(ESI-Trap MS)鉴定两组的蛋白。在NCBI数据库中进行查询以确认蛋白的功能。
考马斯亮蓝染色凝胶检测到207±9个斑点,分子量为14400 - 94000,等电点为3.0 - 10.0。Western印迹显示感染猪的混合血清有7个特异性抗原斑点。7个具有已知功能的抗原中,4个分别归属于细胞骨架肌动蛋白-2(成虫特异性)、原肌球蛋白(囊尾蚴特异性)、AF239799-1膜联蛋白(囊尾蚴特异性)和肌动蛋白-1(囊尾蚴特异性)。
已鉴定出猪带绦虫囊尾蚴的三种特异性抗原。