Singh Sukhwinder, Franks C D, Huang L, Brown-Guedira G L, Marshall D S, Gill B S, Fritz A
Department of Plant Pathology, Kansas State University, Manhattan, KS 66506-5502, USA.
Theor Appl Genet. 2004 Feb;108(4):586-91. doi: 10.1007/s00122-003-1477-8. Epub 2003 Oct 8.
The leaf rust resistance gene Lr41 in wheat germplasm KS90WGRC10 and a resistance gene in wheat breeding line WX93D246-R-1 were transferred to Triticum aestivum from Aegilops tauschii and Ae. cylindrica, respectively. The leaf rust resistance gene in WX93D246-R-1 was located on wheat chromosome 2D by monosomic analysis. Molecular marker analysis of F(2) plants from non-critical crosses determined that this gene is 11.2 cM distal to marker Xgwm210 on the short arm of 2D. No susceptible plants were detected in a population of 300 F(2) plants from a cross between WX93D246-R-1 and TA 4186 ( Lr39), suggesting that the gene in WX93D246-R-1 is the same as, or closely linked to, Lr39. In addition, no susceptible plants were detected in a population of 180 F(2) plants from the cross between KS90WGRC10 and WX93D246-R-1. The resistance gene in KS90WGRC10, Lr41, was previously reported to be located on wheat chromosome 1D. In this study, no genetic association was found between Lr41 and 51 markers located on chromosome 1D. A population of 110 F(3 )lines from a cross between KS90WGRC10 and TAM 107 was evaluated with polymorphic SSR markers from chromosome 2D and marker Xgdm35 was found to be 1.9 cM proximal to Lr41. When evaluated with diverse isolates of Puccinia triticina, similar reactions were observed on WX93D246-R-1, KS90WGRC10, and TA 4186. The results of mapping, allelism, and race specificity test indicate that these germplasms likely have the same gene for resistance to leaf rust.
小麦种质KS90WGRC10中的抗叶锈病基因Lr41和小麦育种系WX93D246-R-1中的一个抗病基因分别从节节麦和圆柱山羊草转移到了普通小麦中。通过单体分析将WX93D246-R-1中的抗叶锈病基因定位到了小麦2D染色体上。对非关键杂交组合的F(2)植株进行分子标记分析确定，该基因位于2D短臂上标记Xgwm210的远端11.2 cM处。在WX93D246-R-1与TA 4186（Lr39）杂交的300个F(2)植株群体中未检测到感病植株，这表明WX93D246-R-1中的基因与Lr39相同或紧密连锁。此外，在KS90WGRC10与WX93D246-R-1杂交的180个F(2)植株群体中也未检测到感病植株。KS90WGRC10中的抗病基因Lr41先前报道位于小麦1D染色体上。在本研究中，未发现Lr41与位于1D染色体上的51个标记之间存在遗传关联。用来自2D染色体的多态性SSR标记对KS90WGRC10与TAM 107杂交的110个F(3)株系群体进行评估，发现标记Xgdm35位于Lr41近端1.9 cM处。用不同的小麦叶锈菌分离株进行评估时，在WX93D246-R-1、KS90WGRC10和TA 4186上观察到了相似的反应。定位、等位性和生理小种特异性测试结果表明，这些种质可能具有相同的抗叶锈病基因。
