Gélis Christelle, Girard Stéphanie, Mavon Alain, Delverdier Maxence, Paillous Nicole, Vicendo Patricia
Laboratoire des IMRCP, UMR 5623 au CNRS, Université Paul Sabatier, 118 route de Narbonne, 31062 Toulouse Cedex 04, France.
Photodermatol Photoimmunol Photomed. 2003 Oct;19(5):242-53. doi: 10.1034/j.1600-0781.2003.00045.x.
UV irradiation can cause cutaneous damage that may be specific according to the wavelength of UV rays. For example, damage from UVB irradiation manifests itself in the form of sunburn cells and enhancement of the expression of p53, while damage from UVA exposure results in an increase in the expression of vimentin. These reactions to UV irradiation were used in this work to evaluate the photoprotective capacities of two sunblock preparations that were applied to the surface of the skin. One sunblock preparation is a UVB absorber containing zinc oxide (ZnO) and titanium oxide (TiO2) exclusively. The other sunblock preparation is a new organo-mineral sunblock containing Tinosorb M, OCM, ZnO and TiO2. Evaluation of the photoprotective capacities of both preparations on hairless rat skin and on in vitro reconstructed human epidermis revealed that they were effective in preventing UVB-induced damage. In contrast, only the organo-mineral sunblock was effective in the prevention of UVA-specific damage such as dermal alterations characterized by the expression of vimentin. Furthermore, our data support the fact that hairless rat skin and in vitro reconstructed human epidermis are a reliable basis for the evaluation of the photoprotective capacities of various sunscreens against UVB and UVA damage.
紫外线照射会导致皮肤损伤，这种损伤可能因紫外线的波长而异。例如，UVB照射造成的损伤表现为晒伤细胞以及p53表达增强，而UVA照射造成的损伤则导致波形蛋白表达增加。在本研究中，利用这些对紫外线照射的反应来评估两种涂抹于皮肤表面的防晒制剂的光保护能力。一种防晒制剂是仅含氧化锌（ZnO）和二氧化钛（TiO2）的UVB吸收剂。另一种防晒制剂是含有Tinosorb M、OCM、ZnO和TiO2的新型有机-矿物防晒剂。对这两种制剂在无毛大鼠皮肤和体外重建人表皮上的光保护能力评估显示，它们能有效预防UVB诱导的损伤。相比之下，只有有机-矿物防晒剂能有效预防UVA特异性损伤，如以波形蛋白表达为特征的皮肤改变。此外，我们的数据支持以下事实：无毛大鼠皮肤和体外重建人表皮是评估各种防晒霜对UVB和UVA损伤光保护能力的可靠依据。
